摘要
人白介素-18(human interleukin 18,hIL18)基因与表皮生长因子受体干扰序列(EGF loop Csequence,EGF)构建IL18-EGF融合基因,利用pET32a/E.coli BL21(DE3)表达IL18-EGF融合基因,通过纯化获得有活性的靶向的IL18-EGF融合蛋白,并进行体外细胞试验评价IL18-EGF融合蛋白的抗肿瘤活性。结果表明IL18-EGF融合蛋白能促进人PBMNC的增殖,诱导KG-1细胞分泌IFN-γ、促进肿瘤抗原诱导的B细胞、NK及其CD4+T细胞的活化。
IL18-EGF fusion gene harboring human epidermal growth factor receptor interference sequence （EGF loop C sequenee,EGF）and human interleukin-18 （IL18）was constructed,and expressed in pET32/E.coli BL21 （DE3）. The antineoplastic activities of IL18-EGF fusion protein were evaluated in vitro. The results showed that IL18-EGF fusion protein could promote proliferation of human peripheral blood mononueleated cells,induce KG-1 cell to secrete IFN-γ and activate B cells ,NK cells and CD4^＋ T cells specific for EGF receptor-expressing tumors.
出处
《科技通报》
北大核心
2009年第2期183-190,201,共9页
Bulletin of Science and Technology
基金
温州市科技项目(Y2006A001)
关键词
IL18-EGF
表达
肿瘤
靶向
IL18-EGF fused protein
expression
tumor
targeting.
