接尘工人血清中TGF-β_1、PⅢNP水平的检测

Detection of TGF-β_1 and PⅢNP Levels in Dust-exposed workers

目的探讨转化生长因子β1(TGF-β1)、Ⅲ型前胶原端肽(PⅢNP)在接尘工人中表达水平的变化及其意义,探讨其作为尘肺早期诊断指标的可能性。方法采用双抗体夹心法,测定69例煤矿接尘工人和45例健康对照者血清中TGF-β1、PⅢNP的表达水平。结果接尘工人血清中TGF-β1、PⅢNP含量分别为(56.85±15.03)、(20.17±23.54)ng/ml,对照组则分别为(18.67±4.48)、(11.72±3.52)ng/ml,接尘组与对照组血清中TGF-β1、PⅢNP含量差异均有统计学意义(P<0.05),尘肺病患者血清中TGF-β1、PⅢNP含量随着接尘工龄的增加而降低,差异有统计学意义(P<0.05);两变量相关分析显示TGF-β1,与PⅢNP呈正相关关系(P<0.05)。结论血清中TGF-β1、PⅢNP表达与接触煤尘有关,细胞因子之间血清水平亦呈正相关,因此,2种细胞因子的异常表达可以作为尘肺病早期诊断的指标之一。

[ Objective] To investigate the expression level changes of transforming growth factorβ1 { TGF-β1 } and procollagen Ⅲ N- terminal peptide （P Ⅲ NP） in dust-exposed workers, and the possibility of being early diagnostic indexes for pneumoconiosis. [ Methods] The serum levels of TGF-β1 and PIIINP in 69 dust-exposed workers and 45 healthy individuals were detected by ELISA. [Results] The serum levels of TGF-β1 and PIIINP in dust-exposed workers were {56.85 ± 15.03） ng/ml, （20. 17 ± 23.54） ng/ml,respectively ; that of control group were { 18.67 ± 4.48 ） ng/ml, （ 11.72 ± 3.52 ） ng/ml, respectively, the differences were significant （P 〈0.05）. The serum levels of TGF-β1 and PIIINP in dust-exposed workers decreased with the increasing age, the difference was significant （ P 〈 0.05 ）. TGF-β1 and P Ill NP showed a positive correlation （ P 〈 0. 05 ） by correlation Analysis. [ Conclusion] Serum expression of TGF-β1 and Pill NP correlates with dust-exposure, and the correlation also exists in the 2 cell factors, therefore abnormal expression of them can be one of the early diagnostic indexes for pneumoconiosis.