杜仲愈伤组织诱导与继代培养研究

Study on Callus Induction and Subculture of Eucommia ulmoides Oliv.

[目的]对杜仲进行愈伤组织诱导及继代培养,建立愈伤组织的培养系统。[方法]以杜仲子叶、幼叶、下胚轴为外植体,接种于不同培养基中诱导和继代。[结果]不同外植体愈伤组织的诱导率高低顺序为:下胚轴>子叶>幼叶。子叶和幼叶在MS+NAA 1.00 mg/L、MS+2,4-D 1.00 mg/L培养基中诱导出的愈伤组织在MS+6-BA 1.00 mg/L+NAA 2.00 mg/L、MS+2,4-D 1.00 mg/L培养基中继代培养为佳;胚轴在MS+NAA 3.00 mg/L、MS+6-BA 1.00 mg/L+NAA 1.00 mg/L和MS+2,4-D 1.00 mg/L培养基中诱导出的愈伤组织在MS+6-BA1.00 mg/L+NAA 2.00 mg/L、MS+6-BA 1.00 mg/L+NAA 1.00 mg/L和MS+2,4-D 1.00 mg/L培养基中继代培养为佳。[结论]杜仲的子叶、幼叶和胚轴可采用多条途径实现愈伤组织的诱导和继代增殖培养,为进一步筛选高产细胞系打下基础。

[ Objective ] The research aimed to study the callus induction and subculture of Eucommia ulmoides Oliv. to establish the callus culture system. [Method] The hypocotyl,cotyledon and young leaf of E. ulmoides Oily.were chose as explants to inoculate in a series of media with hormone at various concentrations. [ Result ] Different explants of E. ulmoides Oliv. could all be induced into callus under suitable hormones. The induction rate in hypocotyl was higher than that of in cotyledon and young leaf.It was best that callus induced from cotyledon and young leaf on media MS ＋ NAA 1.00 mg/L and MS ＋ 2,4-D 1.00 mg/L were inoculated respectively into media MS ＋ 6-BA 1.00 mg/L ＋ NAA 2.00 mg/L and MS ＋ 2,4-D 1.00 mg/L for subculture.And It was best that callus induced from hypocotyl on media MS ＋ NAA 3.00 mg/L, MS ＋ 6-BA 1.00 mg/L ＋ NAA 1.00 mg/L and MS ＋ 2,4-D 1.00 mg/L were inoculated respectively into media MS ＋ 6-BA 1.00 mg/L ＋ NAA 2.00 mg/L, MS ＋ 6-BA 1. 00 mg/L ＋ NAA 1. 00 mg/L and MS ＋ 2,4-D 1.00 mg/L for subculture. [ Conclusion] Callus induction and subculture of E. ulmoides Oily. should be established by various ways, which provide the basis for screening high-yield cell line.