Livin mRNA反义寡核苷酸对人肝癌细胞株HepG-2体外增殖及凋亡的影响

Effects of the Livin mRNA antisense oligodeoxynucleotides on the proliferation and apoptosis of HepG-2 liver cancer cells in vitro

目的探讨LivinmRNA反义寡核苷酸(ASODN)进行肿瘤基因治疗的可行性。方法设计合成嵌合骨架型ASODN及其对照错义寡核苷酸(MSODN),脂质体转染到HepG-2细胞(观察1组和观察2组),同时设未转染者为对照组。采用MTT法检测HepG-2细胞增殖抑制率(IR),电镜、流式细胞仪与吖啶橙/溴化乙锭(AO/EB)细胞染色法检测细胞凋亡水平和形态学变化。结果ASODN在终浓度200nmol/L作用72h时,观察1组HepG-2细胞IR及细胞凋亡数明显高于其他两组。结论以Livin为靶点的ASODN能够促使肿瘤细胞凋亡,具有靶特异性和低毒性,有可能成为抗肿瘤药物开发的新工具。

Objective To investigate the feasibility of Livin mRNA antisense oligodeoxynucleotides （ASODN） in the gene therapy of cancer. Methods Specific Mexid-banckbone oligonuleotides and missense oligonueleotides were synthesized, and then transferred into HepG-2 cells. The inhibition rate （IR） of the proliferation of HepG-2 cells was assayed by MTT method. Electron microscope, flow cytometry （FCM） and acridine orange/ethidium bromide （AO/EB） staining were used to detect the apoptosis and morphologic changes. Results ASODN at a final concentration of 200 nmol/L inhibited the proliferation of HepG-2 and induced apoptosis. Conclusions ASODN with the target of Livin can promote the apoptosis of tumor cells specially and has less toxicity.