摘要
以蝴蝶兰盛花期花梗为外植体,对花梗不同节段、消毒时间、基本培养基、激素浓度配比以及抑制褐化等方面进行了研究。结果表明:第3节段腋芽萌发率最高为95.95%;选用浓度为0.1%升汞消毒15min最佳;1/2MS作为基本培养基腋芽萌发整体效果较好,萌发率达到57.14%;6-BAP5.0mg/L与NAA0.5mg/L激素组合最好,萌发率最高为98.33%。
The article studied different position of pedicel, disinfection requirements, the basic culture medium, hormone concentration and brown restrained, which taken from the Phalaenopsis florescence flower-stalks. The research indicated： the third section of flower-stalks had the highest germination rate which was 95.95% ; the best time of disinfection was 15min by using 0.1% HgCl2; 1/2MS as the basic culture medium was better than others, the highest germination rate was 57.14%. The best hormone concentration combination was 6-BAP 5.0mg/L and NAA0.5mg/L,which had the highest germination rate about 98.33%.
出处
《林业科技开发》
2009年第2期100-103,共4页
China Forestry Science and Technology
关键词
蝴蝶兰
花梗腋芽
萌发率
Phalaenopsis
Flower-stalks
Germination rate
