1型血管紧张素Ⅱ受体基因与中国人冠心病、高血压及糖尿病的关系

THE RELATIONSHIP BETWEEN ANGIOTENSIN Ⅱ TYPE 1 RECEPTOR GENE AND CORONARY HEART DISEASE,HYPERTENSION AND DIABETES MELLITUS IN CHINESE

目的本研究旨在观察１型血管紧张素Ⅱ受体（ＡＧＴＲ１）基因与中国人冠心病（ＣＨＤ）、高血压（ＨＴＮ）及非胰岛素依赖型糖尿病（ＮＩＤＤＭ）的相关情况，并在相关疾病中观察ＡＧＴＲ１基因与血管紧张素Ⅰ转换酶（ＡＣＥ）基因在发病中的相互关系。方法以２７０例ＣＨＤ、ＨＴＮ及ＮＩＤＤＭ单一或不同组合的中国人为对象，用ＰＣＲ／ＤｄｅⅠ酶解检测位于ＡＧＴＲ１基因３′－ＵＴＲ的Ａ１１６６Ｃ变异，并用ＰＣＲ检测ＡＣＥ基因内含子１６的插入／缺失多态标记。结果（１）ＧＡＴＲ１基因与ＣＨＤ相关（１ｏｇｉｓｔｉｃ回归分析，Ｐ＝０．０２）而与ＨＴＮ及ＮＩＤＤＭ无相关；（２）ＡＧＴＲ１基因对ＣＨＤ的参与风险率为１２．４％，比数比４．５５。与ＡＣＥ基因同属ＣＨＤ的独立变异因素（分别为Ｐ＝０．０３２及Ｐ＝０．００２）；（３）ＡＧＴＲ１－Ａ１１６６Ｃ变异不是通过对体脂含量、血脂水平及血压的影响参与ＣＨＤ发病；（４）个体的ＡＧＴＲ１基因及ＡＣＥ基因的联合基因型分析见到ＣＨＤ者与对照者间频率分布有显著差异（Ｐ＝０．０００２）。结论ＡＧＴＲ１基因参与中国人ＣＨＤ发病，并是ＣＨＤ发病的独立风险因素。

Objective To ascertain whether the angiotensin Ⅱ type 1 receptor (AGTR1)gene is associated with coronary heart disease (CHD),hypertension (HTN) and non insulin dependent diabetes mellitus (NIDDM) in Chinese and to observe the interaction of AGTR1 gene and angiotensin I converting enzyme (ACE) gene to the development of the associated disease in Chinese.Methods 270 cases of Chinese with CHD,HTN or NIDDM in single or in different combinations were genotyped for the A1166C variation at the 3′ untranslated region of AGTR1 gene by using PCR/DdeⅠ restriction endonuclease digestion.And also,the insertion/deletion polymorphism at the intron 16 of ACE gene was typed by PCR.Results (1) AGTR1 gene was associated with CHD (logistic regression analysis, P =0 02),but not with HTN and NIDDM;(2)The attributable risk of AGTR1 gene for CHD was 12 5% and the odds ratio was 4 55. Both AGTR1 gene and ACE gene were independent variation factors for CHD ( P =0 032 and P =0 002 respectively);(3) The contribution of AGTR1 gene to the development of CHD was independent of total body adiposity,blood lipid profile and blood pressure;(4) The analysis of individual combined genotypes of AGTR1 gene and ACE gene showed significant difference in frequency distribution between patients with CHD and controls.Conclusion AGTR1 gene contributes to the development of CHD and is an independent risk factor for CHD in Chinese.