摘要
将蛋白饲料源小肽产品按照GB/T18246-2000中酸水解法进行水解及相应的处理,获得氨基酸混合液与2,4-二硝基氯苯(CDNB)衍生化反应后,其中赖氨酸生成二硝基氯苯赖氨酸(DNP-LYS),经日本岛津SPD-10AVG高效液相色谱(HPLC),C18柱分离,UV350nm检测,衍生后氨基酸混合物得到较好的分离,赖氨酸最低检出量2μg。
Small peptides products from feed protein source had been made to hydrolyze and deal according to the acid hydrolysis method of GB/T 18246-2000, and intermixture of amino acid was obtained, obtained intermixture of amino acid was mixed with 2,4-dinitrochlorobenzene （30 mg/mL） to progress chemical reaction, Lysine belong to intermixture of amino acid became dinitrochlorobenzene- Lysine; Finally, we used high performance liquid chromatography （HPLC） of Janpan SPD-10AVG, C18 column to separate, UV350 nm detection, intermixture of amino acid ramification could be preferable separated, lysine＇s minimum detectable amount 2μg.
出处
《乳业科学与技术》
2009年第2期78-80,共3页
JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY
基金
黑龙江省教育厅博士类专项科研基金(HLJBSDJI2004-15)
东北农业大学创新团队项目(CXT005-1-2)
关键词
小肽产品
赖氨酸
高效液相色谱
small peptides products
lysine
high performance liquid chromatography
