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重组H5N3亚型禽流感细胞毒灭活疫苗免疫佐剂效果的评价 被引量：1

Evaluation of adjuvant effect on rH5N3 subtype avian influenza virus cell cultured vaccine in SPF chickens and ducks
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摘要 为评价佐剂对疫苗免疫效果的影响,本试验以反向遗传学技术拯救的重组H5N3亚型禽流感病毒为种毒,对国产矿物油以及赛比克公司提供的MontanideTM ISA 70MVG(简称70M)和MontanideTM ISA 206 VG(简称206)3种佐剂制备的灭活疫苗进行了SPF鸡免疫攻毒试验。结果表明国产矿物油佐剂组与70M佐剂组都能对SPF鸡100%保护,206佐剂组仅能保护40%,攻毒的SPF鸡发病,但不死亡;从抗体水平上比较发现70M佐剂组稍好于国产矿物油佐剂组,但两组之间差异不显著,而这两组与206佐剂组差异极显著。这3种佐剂组免疫鸭均能够诱导产生较高的抗体水平,70M佐剂组优于国产矿物油佐剂组和206佐剂组,但3者差异不显著。用70M系的8种佐剂MontanideTM ISA 70 essai Mi(i=1-8)(简称70Mi)佐剂乳化的疫苗免疫SPF鸡,结果表明70M佐剂组效果最好,攻毒后免疫鸡不排毒,而且无临床症状;统计学分析显示70M佐剂组与所有70Mi佐剂组差异均显著。这些结果表明70M佐剂可以作为新型疫苗佐剂用于禽流感疫苗的制备。 In order to evaluate the effect of different adjuvant on cell culture vaccine of avian influenza virus rH5N3 subtype, three types of adjuvant including domestic mineral oil, MontanideTM ISA 70M VG （abbr. 70M） and MontanideTM ISA 206 VG （abbr. 206） obtained from SEPPIC Co. were tested. SPF chickens were immunized with inactivated avian influenza virus rH5N3 subtype in respective adjuvant and challenged. The results showed that both mineral oil adjuvant group and 70M adjuvant group provided 100% protection efficiency, but 206 adjuvant group only provided 40% protective efficiency. No significant differences between the mineral oil adjuvant group and the 70M adjuvant group were found.
作者 刘春国 刘明 万春和 陈浩 李洪涛 孙恩成 杜金玲 刘大飞
机构地区 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/农业部动物流感重点实验室/国家禽流感参考实验室/OIE禽流感参考实验室
出处 《中国预防兽医学报》 CAS CSCD 北大核心 2009年第3期217-222,共6页 Chinese Journal of Preventive Veterinary Medicine
基金 科技支撑计划(2006BAD06A05) 黑龙江省自然科学基金(ZJN0702-02)
关键词 禽流感 H5N1亚型 细胞苗 佐剂 avian influenza subtype H5N1 cell cultured vaccine adjuvant
分类号 S859.797 [农业科学—临床兽医学]
  • 相关文献

参考文献14

  • 1Webster R G, Bean W J, Gorman O T, et al. Evolution and ecology of influenza A virus [J]. Microbiol Rev, 1992, 56: 152-179.
  • 2Li K S, Guan Y, Wang J, et al. Genesis of a highly pathogenic and potentially pandemic H5N1 influenza virus in eastern Asia [J]. Nature, 2004, 430: 209-213.
  • 3Ungchusak K, Auewarakul P, Dowell S F, et al. Probable person- to-person transmission of avian influenza A (H5N1) [J]. N Engl J Med, 2005, 352: 333-340.
  • 4刘明,张云,刘春国,潘蔚绮,刘超男,杨涛.利用反向遗传学技术构建H5亚型禽流感高产疫苗株[J].生物工程学报,2006,22(5):720-726. 被引量：17
  • 5Liu M, He S, Walker D, et al. The influenza virus gene pool in a poultry market in South central china [J]. Virology, 2003, 305: 267-275.
  • 6刘明,刘春国,张云,杨涛.N3亚型禽流感病毒NA基因在昆虫细胞中的高效表达[J].动物医学进展,2005,26(10):50-54. 被引量：5
  • 7刘超男,刘明,张云,刘春国,高雪丽.H5N1亚型禽流感病毒的鸭致病性研究[J].中国农业科学,2006,39(2):412-417. 被引量：25
  • 8Harold F, Stills J. Adjuvants and antibody production: dispelling the myths associated with freund's complete and other adjuvants [J]. ILAR Journal, 2005, 46(3): 280-293.
  • 9Johnston B A, Eisen H, Fry D. An evaluation of several adjuvant emulsion regimens for the production of polyclonal antisera in rabbits [J]. Lab Anim Sci, 1991, 41: 15-21.
  • 10Leenaars P, Hendfiksen C, Angulo A, et al. Evaluation of several adjuvants as alternatives to the use of Freund's adjuvant in rabbits [J]. Vet Immunol Immunopath, 1994, 40: 225-241.

二级参考文献52

  • 1Saif Y M,Barnes H J,Gilsson J R,Fadley A M,MacDougald L R,Swayne D E.Diseases of Poultry.11th edition.Ames:Iowa State University Press,2003:135-160.
  • 2Perkins L E,Swayne D E.Pathogenicity of a Hong Kong-origin H5N1 highly pathogenic avian influenza virus for emus,geese,ducks,and pigeons.Avian Disease,2002,46:53-63.
  • 3Reed L J,Muench H.A simple method for estimating fifty percent endpoints.American Journal of Hygiene,1938,27:493-497.
  • 4Munch M,Nielsen L,Handberg K,Jorgensen P.Detection and subtyping (H5 and H7) of avian type A influenza virus by reverse transcription-PCR and PCR-ELISA.Archieves of Virology,2001,146:87-97.
  • 5Chen H,Deng G,Li Z,Tian G,Li Y,Jiao P,Zhang L,Liu Z,Webster R G,Yu K.The evolution of H5N1 influenza viruses in ducks in southern China.The Proceedings of the National Academy of Science of the USA,2004,101:10452-10457.
  • 6Sturm-Ramirez K,Ellis T,Bousfield B,Bissett L,Dyrting K,Rehg J,Poon L,Guan Y,Peiris M,Webster R G.Reemerging H5N1 influenza viruses in Hong Kong in 2002 are highly pathogenic to ducks.Journal of Virology,2004,78:6988-6994.
  • 7Webster R G,Yakhno M,Hinshaw V S,Bean W J,Murti K G.Intestinal influenza:replication and characteri-zation of influenza viruses in ducks.Virology,1978,84:268-278.
  • 8Capua I,Mutinelli F.Mortality in Muscovy ducks (Cairina moschata) and domestic geese (Anser anser var.domesticus) associated with natural infection with a highly pathogenic avian influenza virus of H7N1 subtype.Avian Pathology,2001,30:179-183.
  • 9Perkins L E,Swayne D E.Susceptibility of laughing gulls to H5N1 and H5N3 high pathogenic avian influenza viruses.Avian Disease,2002,46:877-885.
  • 10Subbarao K,Klimov A,Katz J,Regenery H,Lim W,Hall H,Perdue M,Swayne D,Bender C,Huang J,Hemphill M,Rowe T,Shaw M,Xu X,Fukuda K,Cox N.Characterization of an avian influenza A (H5N1) virus isolated from a child with a fatal respiratory illness.Science,1998,279:393-396.

共引文献44

同被引文献10

  • 1张晓霁,刘明,刘春国,杨涛,张云.H5N1亚型禽流感病毒HA基因在昆虫细胞中的表达及其生物活性鉴定[J].中国生物工程杂志,2007,27(3):42-46. 被引量：6
  • 2Neumann G,Kawaoka Y. Host range restriction and pathogenicity in the context of influenza pandemic[J].Emerging Infectious Diseases,2006.881-886.
  • 3Kang S M,Song J M,Compans R W. Novel vaccines against influenza viruses[J].Virus Research,2011,(1-2):31-38.
  • 4Quan F S,Huang C,Compans R W. Virus-like particle vaccine protetive immunity against homologous and heterologous strains of influenza virus[J].Journal of Virology,2007,(07):3514-3524.
  • 5Galarza J M,Latham T,Cupo A. Virus-like paticle (VLP) vaccine conferred complete protection aginst a lethal influenza virus challenge[J].Viral Immunology,2005,(01):244-251.doi:10.1089/vim.2005.18.244.
  • 6Bright R A,Carter D M. Cross-clade protective immune reponses to influenza viruses with H5N1 HA and NA elicited by an influenza virus-like particle[J].PLoS One,2008,(01):e1501.
  • 7Song J M,Hossain J,Yoo D G. Protective immunity against H5N1 influenza virus by a single dose vaccination with virus-like particles[J].Virology,2010,(01):165-175.
  • 8Chang Ro-lin,Lai Su-yuan,Chang Pao-chun. Production of immunogenic one-component avian H7-subtype influenza[J].Process Biochemistry,2011.1292-1298.
  • 9Kang S M,Song J M,Quan Fu-shi. Influenza vaccines-based on virus-like particles[J].Virus Research,2009,(02):140-146.
  • 10张树梅,黄海碧,颜丕熙,滕巧泱,闫丽萍,徐大伟,呼和巴特尔,李泽君.影响A型流感病毒样颗粒包装及释放的关键结构蛋白研究[J].中国农业科学,2011,44(13):2823-2829. 被引量：5

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中国预防兽医学报
2009年 第3期

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