地塞米松对慢性支气管炎肺泡巨噬细胞释放TNF_αTXB_2和6-keto-PGF_(1α)的影响

EFFECTS OF DEXAMETHASONE ON THE TNFα,TXB2 AND 6KETOPGF1α RELEASE BY ALVEOLAR MACROPHAGES IN CHRONIC BRONCHITIS

通过支气管肺泡灌洗（ｂｒｏｎｃｈｏａｌｖｅｏｌａｒｌａｖａｇｅ，ＢＡＬ）从１３例慢性支气管炎缓解期病人和１１例正常人获得的肺泡巨噬细胞（ａｌｖｅｏｌａｒｍａｃｒｏｐｈａｇｅｓ，ＡＭ）加入地塞米松４ｍｇ／Ｌ及脂多糖（ｌｉｐｏｐｏｌｙｓａｃｃｈａｒｉｄｅ，ＬＰＳ）１ｍｇ／Ｌ体外培养２４小时。结果显示两组ＡＭ合成分泌的ＴＮＦα、ＴＸＢ２和６－ｋｅｔｏ－ＰＧＦ１α呈下降趋势，两组间ＡＭ合成分泌的这些活性分子均呈显著差异性（Ｐ＜０．０５）。表明地塞米松抑制ＡＭ的活化，可能慢支病人呼吸道内ＡＭ功能相对亢进。

Alveolar macrophages (AM) obtained from 13 chronic bronchitis patients and 11 normal subjects by bronchoalveolar lavage were cultured with 4 mg/L dexamethasone and 1 mg/L lipopolysaccharide (LPS) for 24h in vitro.The results showed that AMs from the two groups elaborated less TNFα,TXB2 and 6ketoPGF1α than the control,there is a significant difference between AMs from the two groups in producing the molecular entities (P<005).Our findings indicate that dexamethasone inhibits the activation of AMs,and AMs of respiratory tract in chronic bronchitis patients may have a considerable hyperfunction.