摘要
Nogo和其受体相互作用可能在抑制神经再生中发挥着重要作用.运用免疫细胞化学方法,本研究观察了Nogo受体(NgR)在中菊头蝠脑中的分布.结果显示NgR在中菊头蝠脑皮层各层均有表达.在海马,NgR主要分布在CA1、CA3和DG区的神经细胞胞膜、胞质或/和突起上.杏仁核、丘脑、室旁核、视上核、视交叉上核等也有NgR阳性神经细胞着色.在脑的白质,轴突着色明显.小脑的分子层、Purkinje细胞层和颗粒细胞层均有NgR免疫阳性反应,其中阳性反应的颗粒细胞最多.这些提示NgR可能介导其配基对中菊头蝠脑多个区域的神经细胞起作用.
An interaction of Nogo with NgR has been suggested to play an important role in limiting axonal growth. By means of immunocytochemistry, the present study investigated the distribution of NgR in the brain of Rhinolophus affinis. The results showed that the NgR protein appeared to be present in all layers of the brain neocortex. In the hippocarnpus, NgR immunoreactivity was widely located in neurons, mainly concentrated in CA1, CA3 of the stratum pyrarnidale and dentate gyms. Neurons in the amygdala, thalamus, paraventricular nucle, supraopticus and suprachiasmaticus aiso expressed NgR protein. The labeled neuronal cells exhibited immunoreactivity mainly in cytoplasmic membranes, cell bodies and/or neuropil. In white matter, axonal staining for NgR is prominent. In the cerebellum, immunoreactivity was detected in the molecular layer, Purkinje cell layer and granule cell layer. There were numerous granule cells and a few of Purkinje cells stained for NgR. These indicated that the NgR may mediate effects of its ligands on the neuronal cells in various brain regions of Rhinolophus affinis.
出处
《安徽师范大学学报（自然科学版）》
CAS
北大核心
2009年第1期64-68,共5页
Journal of Anhui Normal University(Natural Science)
基金
Supported by NSFC(30470537)
NSF of Anhui Education Committee(ZD2008006-1)
NSF of Anhui Provincial Laboratory of the Conservation and Exploitation of Biological Resources
NSF of Anhui Key Laboratory of Biotic Environment and Ecological Security
The Open Foundation of the Key Laboratory of Molecular Evolution and Biodiversity of Anhui Normal University
