肿瘤坏死因子-β基因多态性与特发性扩张性心肌病的相关性分析

The association of TNF-β polymorphism with idiopathic dilated cardiomyopathy （IDC） in Northern Chinese Han population

目的探讨肿瘤坏死因子（TNF）-β基因型的多态性与特发性扩张性心肌病（IDC）的相关性。方法在吉林大学第一临床医院，选择86例IDC患者为IDC组、95例体检健康者为对照组，用ELISA法检测血清TNF—β蛋白水平，采用聚合酶链反应-限制性片段长度多态性（PCR—RFLP）分析法检测TNF—β基因多态性变化。结果IDC组TNF—β蛋白水平[（1．876±1．013）μg／L]高于对照组[（1．018±0．645）μg／L]，组间比较差异有统计学意义（t=2．67，P〈0．01）；TNFβ＊2等位基因频率[63．4％（109／172）]高于对照组比[47．9％（91／190）]，组间比较差异有统计学意义（Χ^2=6．78，P〈0．05）．其比值比（OR）值为1．88。结论TNFβ＊2等位基因的多态性与IDC的易感性有关，可能是IDC的易感基因之一。

objective To study the relationships between TNF-β gene type in idiopathic dilated cardiomyopathy （IDC）. Methods Eighty-six IDC patients were chosen as IDC group and 95 cases unrelated healthy people as control group in the First Hospital of Jilin University in 2008. The levels of TNF-β protein were detected with Enzyme- linked immunosorbent assay（ELISA）. TNF-β polymorphism were analyzed by polymerase chain reaction-restriction fragment length polymorphisms（PSR-RFLP）. Results The levels of TNF-β protein in IDC patients[ （1.876 ± 1.013） μg/L ] were significantly higher than controls[ （1.018 ± 0.645 ）μg/L], the difference being statistically significant（t = 2.674 ,P 〈 0.01 ）. The frequency of TNFβ＊2 genotypes in IDC patients[63.4%（ 109/172] was significantly higher than controls[47.9%（91/190） ], the difference being statistically significant（OR = 1.88,Χ62 = 6.78, P 〈 0.05）. Conclusions The TNFβ＊2 allele might related to idiopathic dilated cardiomyopathy （IDC）, can also be considered as one of susceptible genes of IDC