瞬时感受电位基因家族成员TRPV4在大鼠睾丸组织中的表达

Expression of a subfamily member of transient receptor potential gene(TRPV4) in rat's testis

背景:瞬时感受电位(transient receptor potential,TRP)通道在人体遍布多种组织和细胞,并参与人体几乎所有的生理功能和许多病理变化。目的:观察瞬时感受电位基因亚家族成员TRPV4在大鼠睾丸组织中的表达。设计、时间及地点:单一样本观察。实验于2007-03/2008-04在广东省人民医院医学研究中心完成。材料:SD大鼠5只;TRPV4引物由上海生物工程技术服务有限公司合成。方法:选用SD大鼠,取20mg睾丸组织,用RNAprep_动物组织/细菌总RNA提取试剂盒提取总RNA,取1μg总RNA,用于合成cDNA。主要观察指标:用常规反转录-聚合酶链反应和免疫组织化学染色检测TRPV4在大鼠睾丸组织中的表达。结果:常规反转录-聚合酶链反应方法检测到在TRPV4的泳道中可见一条带,大小与期望长度符合;免疫组织化学染色可见TRPV4阳性片中较成熟的生精细胞和支持细胞为黄褐色。结论:TRPV4在睾丸组织上有表达,主要表达在较成熟的生精细胞和支持细胞上。

BACKGROUND： The transient receptor potential （TRP） protein channel distribute many kinds of tissue and cell in human body, which take part in all kind of physiologic function and pathological changes. OBJECTIVE： To study the expression of TRPV4, a subfamily member of TRP gene in rat＇s testis. DESIGN, TIME AND SETTING： A single sample observation was performed at the Research Center of Guangdong General Hospital from March 2007 to April 2008. MATERIALS： Five healthy male SD rats were collected in the study. The primer of TRPV4 was constructed by Shanghai Sangon Biological Engineering Technology ＆ Services Co., Ltd. METHODS： A total of 20 mg testes tissues of rats were extracted total RNA with total RNA extraction kit, 1 μg total RNA was obtained and to synthesis cDNA. MAIN OUTCOME MEASURES： The expression of TRPV4 in testis was detected by RT-PCR and immunohistochemistry staining. RESULTS： A strap which in line with the expected length was detected in the track of TRPV4 by RT-PCR. Spermatogenic cell and Sertoli cell was stained cinnamomeous by Immunohistochemistry. CONCLUSION： TRPV4 expresses on the rats＇ testes, especially in spermatogenic cell and Sertoli cell.