软骨利胶囊对骨关节炎小鼠股四头肌收缩蛋白mRNA表达的影响

Influence of Ruanguli capsule on mRNA expression of quadriceps femoris contractile protein in mice with osteoarthritis

背景:骨关节炎的病理改变涉及多种关节构成组织,其中骨骼肌的地位日渐引起研究者关注。目的:通过观察骨关节炎C57小鼠骨骼肌收缩蛋白α-肌动蛋白与肌球蛋白重链基因表达状况,探讨柔肝中药软骨制剂利胶囊对收缩蛋白基因表达的影响。设计、时间及地点:随机对照动物实验,于2004-09/2007-01在上海市中医药研究院骨伤科研究所完成。材料:选取7周龄清洁级C57小鼠18只,雌雄各半,体质量(20±5)g。软骨利胶囊(柔肝中药制剂),由上海中医药大学中药标准化中心提供。方法:18只C57小鼠按随机数字表随机分为正常组、模型组、软骨利组,每组6只。除正常组外,对小鼠进行运动负荷训练造模。软骨利组给予0.2mL软骨利溶液(约含2mg软骨利)灌胃5周,正常对照组和模型组给予0.2mL生理盐水灌胃5周。主要观察指标:应用反转录-聚合酶链反应检测股四头肌肌α-actin、肌球蛋白重链4种亚型(Ⅰ,Ⅱa,Ⅱd/x,Ⅱb)mRNA表达水平。结果:与空白组比较,模型组α-actin mRNA显著低表达(P<0.05),肌球蛋白重链Ⅱa,肌球蛋白重链Ⅱd/x mRNA低表达(P<0.05)。与模型组比较,软骨利干预组α-actin与多种肌球蛋白重链亚型mRNA表达水平上调(P<0.05)。结论:柔肝中药制剂软骨利能够促进骨骼肌收缩蛋白基因表达,提示其可改善骨关节炎动物骨骼肌肌力。

BACKGROUND： Multi-tissues are involved in osteoarthritis pathology, and the effect o4 skeletal muscle in the pathogenesis has attracted more attention. OBJECTIVE： To investigate the mRNA expression of α -actin, myosin heavy chain （MHC） isoforms in C57 mice with osteoarthritis, in addition, to explore the effect of Ruangufi （liver-softening formulation） on the treatment of osteoarthritis. DESIGN, TIME AND SETTING： A randomized control animal experiment was performed at the Department of Orthopedics and Traumatology, Tradition Chinese Medicine Academy of Shanghai between September 2004 and January 2004. MATERIALS： A total of 18 healthy, 7 weeks old, clean grade, C57 mice, with body weight of （20±5） g, half male and half female, were selected. Ruangufi was provided by Standardization Center of Tradition Chinese Medicine Academy of Shanghai. METHODS： Eighteen C57 mice were randomly divided into blank control, model, and Ruangufigroups, with 6 animals in each group. All mice were prepared exercise load models exception of the blank control group. Mice in the Ruanguli group were intragastrically administrated 0.2 mL Ruanguli solution （containing 2 mg Ruanguh） for 5 weeks. Animals in the other two groups were administrated 0.2 mL physiological saline fro 5 weeks. MAIN OUTCOME MEASURES： The mRNA expression level of a -actin and MHC isoforms in quadriceps femoris was tested by RT-PCR. RESULTS： The level of α -actin mRNA in model group was lower than that of the normal control group （P 〈 0.05）, with low expression of MHC Ⅱ a, and MHC Ⅱ d/x mRNA （P 〈 0.05）. Compared with the model group, a -actin and MHC isoforms was up-regulated in the Ruanguli group （P 〈 0.05）. CONCLUSION： Ruangufi can promote the gene expression of contractile protein, which is capable for strength the myodynamia of skeletal muscle.