摘要
目的探讨肠血管活性多肽(VIP)或生长抑素(SST)对多器官功能障碍综合征(MODS)大鼠小肠黏膜地址素黏附分子-1(MAdCAM-1)表达的影响及其对MODS防治的意义。方法36只雄性Wistar大鼠随机分为6组(每组6只),包括对照组(正常大鼠)、VIPI组和SST1组(分别经VIP和SST处置的正常大鼠)、MODS组(MODS大鼠)、VIP2组和SST2组(分别经VIP和SST处置的MODS大鼠)。采用肠缺血再灌注方法制作MODS大鼠(出现全身炎症反应,〉2个器官功能障碍)模型。VIP或SST以0.2ρmol·g^-1·h^-1静脉输入和0.25ρmol/g腹腔注入大鼠体内。各组收集的肠淋巴细胞用^51Cr标记后回输入大鼠体内,γ计数器测定其在肠相关淋巴组织(GALT)的数量分布;Western blot测定各组小肠黏膜MAdCAM-1的表达;组织化学法观察各组小肠黏膜组织学变化。数据采用t检验进行分析。结果VIP1组和SST1组小肠弥散淋巴组织MAdCAM-1表达的峰浓度值分别为(157.67±2.52)、(154.33±3.22),Peyer's结为(136.00±1.00)、(137.00±1.00),较对照组[(165.33±1.53)、(152.67±2.31)]无显著改变(P〉0.05);归巢至小肠弥散淋巴组织^51Cr-细胞量占总^51Cr-细胞量的1.04%±0.59%、1.01%±0.83%,较对照组(1.07%±0.61%)无显著改变(P〉0.05);Peyer's结为1.83%±0.90%、1.56%±0.64%,显著低于MODS组[(3.85%±2.02%),P〈0.05]。VIP2组和SST2组小肠弥散淋巴组织MAdCAM-1表达的峰浓度值分别为(158.00±2.65)、(154.33±1.53),Peyer's结为(156.33±1.53)、(151.33±2.31),较MODS组[(175.33±2.52)、(173.00±2.65),P〈0.05];归巢至小肠弥散淋巴组织^51Cr-细胞量占总^51Cr-细胞量的1.58%±0.42%、1.45%±0.26%,Peyer's结为2.14%±1.49%、0.81%±0.35%,显著低于MODS组[(3.23%±1.69%)、(5.04%±1.23%),P〈0.05],并伴有肠黏膜组织学损害的减轻。结论增加MODS大鼠血循环中的VIP或SST,可通过抑制肠黏膜MAdCAM-1表达,减少肠淋巴细胞归巢至GALT,减轻MODS时肠黏膜的炎性损伤。
Objective To investigate the effects of somatostatin (SST) or vasoactive intestinal peptide (VIP)on the expression of MAdCAM-1 in the intestinal mucosa of rats with multiple organ dysfunction syndrome (MODS) and its significance of prevention and treatment of MODS. Method Thirty six Wistar male rats were randomly divided into 6 groups (6 rats in each group), including control group,VIP group 1 and SST groupl (rats treated with VIP and SST respectively), MODS group (rats with MODS), VIP group 2 and ssr group 2(MODS rats treated with VIP and SST respectively). The rat model of MODS (system inflammnatory response syndrome, 〉 2 organs dysfunction) was established by occlusion of superior mesenteric arteries. 0.2 ρmol·g^-1· h^-1 VIP or SST by intravenous injection combined with 0.25 ρmol/g VIP or SST by intraperitoneal injection were injected into rats. In each group, intestinal lymphocytes from rats labeled with ^51Cr were infused into rat veins and were quantified with γcounter in GALT. The expression of MAdCAM-1 in the intestinal mucosa was measured by western blot. Inflammation in the intestinal mucosa was evaluated with histological sections. Student's t test was used to assess difference between the experiment group and the control group. Results In VIP group 1 and SST group 1, the peak values of MAdCAM-1 expression in diffusive lymphatic tissue of small intestinal were 157.67 ± 2.52 and 154.33 ± 3.22, and those in Peyer patches were 136.00 ± 1.00 and 137.00 ± 1.00. There were no significant difference when compared with control group (165.33 ± 1.53, 152.67 ± 2.31, P 〉 0.05 ). The percentage of ^51Cr-lymphocytes in diffusive lymphatic tissue of small intestine( 1.04% ± 0.59%, 1.01% ± 0.83%) showed no significant difference from control group (1.07% ± 0.61%, P 〉 0.05), and those in Peyer patches (1.83% ± 0.90%, 1.56% ± 0.64% ) were significantly less than control group (3.85% ± 2.02%, P 〈 0.05). In VIP group 2 and SST group 2, the peak values of MAdCAM-1 expression in diffusive lymphatic tissue of small intestinal ( 158.00 ± 2.65,154.33± 1.53) and Peyer patches (156.33 ± 1.53, 151.33 ± 2.31) were significantly less than MODS group (175.33 ± 2.52, 173.00 ± 2.65, P 〈 0.05). The percentage of ^51Cr-lymphocytes in diffusive lymphatic tissue of small intestine ( 1.58 % ± 0. 42 %, 1.45 % ± 0. 26 % ) and Peyer patches (2. 14 % ± 1.49 %, 0. 81% ± 0.35%) were significantly less than MODS group (3.23% ± 1.69%, 5.04% ± 1.23%, P 〈0.05) and the severe histopathological damage in intestine was relieved. Conclusions VIP or SST reduced intestinal lymphocytes homing to GALT in rats with MODS through suppressing the expression of MAdCAM-1, and attenuated the inflammatory injure in the intestinal mucosa.
出处
《中华急诊医学杂志》
CAS
CSCD
北大核心
2009年第3期257-261,共5页
Chinese Journal of Emergency Medicine
基金
基金项目:国家自然科学基金资助项目(30170875)
关键词
肠血管活性多肽
生长抑素
黏膜地址素黏附分子-1
多器官功能障碍综合征
Vasoactive intestinal polypeptide
Somatostatin
Mucosal addressin cell adhesion molecule-1
Multiple organ dysfunction syndrome