两种新型支架材料的细胞相容性

Cell compatibility of two novel scaffold materials

背景:利用骨组织工程原理,通过观察成骨细胞在生物材料上的形态、测定成骨细胞在生物材料上的黏附率等方法,可评估生物支架材料的生物相容性。目的:通过扫描电子显微镜下细胞-生物材料的观察和细胞-生物材料黏附率的测定,探讨骨组织工程材料的细胞相容性。设计、时间及地点:动物实验,成骨细胞形态学观察,于2007-02/2007-09在华南理工大学材料科学与工程学院生物材料研究所教育部重点实验室完成。材料:健康SD乳鼠30只,2种支架材料分别是:复合海藻酸钠和聚乳酸羟基乙酸的磷酸钙骨水泥以及卵磷脂改性聚乳酸羟基乙酸/生物活性玻璃复合支架。方法:取SD乳鼠颅骨成骨细胞,经体外培养、扩增,与两种新型可降解生物活性支架材料进行复合,通过扫描电镜观察细胞形态并测定细胞在生物材料上的黏附率。主要观察指标:观察接种于生物材料表面上的成骨细胞形态、黏附情况,对生物材料生物相容性进行描述。结果:生物活性材料与细胞复合后,材料出现了矿化,同时在海藻酸钠/聚乳酸羟基乙酸磷酸钙骨水泥通过扫描电镜观察发现其上游生长形态良好的成骨细胞;成骨细胞在生物卵磷脂改性聚乳酸羟基乙酸生物活性玻璃上的黏附率最高达到了95.2%。结论:从细胞形态和细胞黏附率上看,海藻酸钠/聚乳酸羟基乙酸磷酸钙骨水泥和生物卵磷脂改性聚乳酸羟基乙酸生物活性玻璃具有较好的生物相容性。

BACKGROUND： Under the theory of bone-tissue engineering, biocompatibility of biomaterial is evaluated by observing the morphous and determining the adhesion rate of osteoblasts on biomaterials. OBJECTIVE： To explore cell compatibility of bone tissue-engineered materials by observing cell-biomaterial and determining cell-biomaterial adherent rate using a scanning electron microscope. DESIGN, TIME AND SETTING： The osteoblast morphology animal experiment was conducted at the Key Laboratory of Education Ministry, Institute of Biomaterial, College of Material and Engineering, South China University of Technology from February to September 2007, MATERIALS： A total of 30 healthy Sprague Dawley neonatal rats were used. Calcium phosphate bone cement compounded sodium alginate and poly lacticoglycolic acid （PLGA） and lecithin reshaped PLGA/bio-active-glass scaffold. METHODS： Osteoblasts were taken from the cranium of Sprague Dawley neonatal rats. After culture and amplification in vitro, osteoblasts were compounded to the novel degradable bio-active-material scaffolds. Cell morphology was observed using a scanning electron microscope, and adherent rate of osteoblasts on biomaterials was determined. MAIN OUTCOME MEASURES： Osteoblast morphology and adhesion on the surface of biomaterials were observed. Biocompatibility of biomaterials was described. RESULTS： Mineralization was found following cell-biomaterial compounds. Meanwhile, some good appearance osteoblasts were discovered on sodium alginate/PLGA phosphate calcium bone cement by using the scanning electron microscope. The maximum adhesion rate of osteobiasts on PLGA-Lecithine-Bioactive-glass was 95.2%. CONCLUSION： According to cell morphology and the adherent rate, sodium alginate/PLGA phosphate calcium bone cement and the PLGA-Lecithine-Bioactive-glass have good biocompatibility.