摘要
目的为预防输血后乙型肝炎,是否有必要在献血者中加做抗-HBc筛查以及采取何种取舍标准?为此,对常规筛查合格的献血者血液进行了抗-HBc滴度与HBVDNA相关性的研究。方法用免疫-套式PCR法检测了297份不同滴度组抗-HBc阳性血液的HBVDNA。结果在4份抗-HBc滴度≥1128的血液中检出1例HBVDNA,而在另75份抗-HBc滴度<1128的血液中未检出,HBVDNA与抗-HBc滴度之间有显著相关性。在218份抗-HBc/抗-HBs双阳血液及50份抗-HBc阴性血液中未检出HBVDNA。结论仅含高滴度单项抗-HBc的血液可能具有传染性;HBVDNA在抗-HBc阳性血液中的总检出率低,仅为0.34%(1/297)。建议将有限的资金首先用于进一步提高现有献血者HBsAg检测试剂的灵敏度(目前为1ng/ml),或许更为有效。
Objective To study if it is necessary to screen for core antibody against hepatitis B (anti-HBc) to prevent post transfusion hepatitis B and what is its criteria for screening. Methods Relationship between the titer of anti-HBc and HBV DNA in blood from qualified donors by routine testing was conducted. HBV DNA was detected in 297 units of blood with anti-HBc at various titers by immuno nested polymerase chain reaction (RT PCR). Results HBV DNA was detected in one of four units of blood with positive anti HBc at a dilution of equal to or greater than 1∶128, and no HBV DNA was detected in other 75 units of blood with a dilution of anti HBc less than 1∶128. There was significant relationship between HBV DNA and the titer of anti-HBc. No HBV DNA was detected in 218 units of blood with both positive anti-HBc and anti-HBs and in 50 units with negative anti-HBc. Detection rate of HBV DNA in blood with positive anti-HBc was only 0.34% (1/297). Conclusion Blood containing higher titer of single anti-HBc may be infectious. It is suggested that sensitivity of the reagents for detecting HBsAg currently used (1 ng/ml) be improved with the limited budget.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
1998年第1期7-9,共3页
Chinese Journal of Preventive Medicine
基金
北京市青年科技骨干培养基金
北京市卫生局科研基金
关键词
供血员
乙型肝炎病毒
抗体
聚合酶链反应
Blood donor Hepatitis B antibodies DNA Polymerase chain reaction