生物学标志在产肠毒素性大肠埃希菌同源克隆鉴定中的应用

Application of Biomarkers in Homologous Clone Identification of Enterotoxigenic Escherichia Coli

目的研究多方法、多生物学标志在产肠毒素性大肠埃希菌同源克隆鉴定中的作用。方法在毒力基因检测和分型基础上，首次采用质粒分析法结合多位点酶电泳法，对ＥＴＥＣ同源克隆进行了综合研究。结果７个毒力基因型的２０６株散发ＥＴＥＣ菌株经质粒图谱分型后，共可分成９３个质粒型，有１４０株ＥＴＥＣ具有独特的基因型；进一步用多位点酶电泳分型，２０６株ＥＴＥＣ可被分成１４８个ＥＴ型，３种方法综合，共有１９０株ＥＴＥＣ具有独特的基因型。结论多方法、多生物学标志在同源克隆鉴定中能提供更多、更可靠的信息。

Objective To study the application of multiple molecular biotechniques and multi biomarkers in homologous clone identification of enterotoxigenic Escherichia coli (ETEC). Methods On the basis of detection and typing for toxigenic genes, plasmid analysis combined with multilocus enzyme electrophoresis (MEE) was used for the first time in the comprehensive research on ETEC homologous clone. Results Two hundred and six strains of ETEC could be classified into 93 plasmid types with plasmid profiling, and 140 strains with unique genotype. Those 206 strains of ETEC could be classified into 148 electrophoresis types by MEE further, and altogether 190 strains with unique genotype, by combination of three methods. Conclusion Multiple molecular biotechniques and multi biomarkers can provide much more and more reliable information in homologous clone identification.