二硫化碳对大鼠神经递质及其代谢产物的影响

Effects of Carbon Disulfide on Neurotransmitter and Its Metabolites in Rats

目的探讨二硫化碳ＣＳ２的神经毒作用机理。方法应用高效液相色谱法，观察大鼠染毒ＣＳ２（０、１２００、２４００ｍｇ／ｍ３）２个月后，脑组织单胺类递质、氨基酸类神经递质及其代谢产物的改变。结果ＣＳ２染毒可使大鼠纹状体中３－甲氧－４－羟基扁桃酸含量下降，多巴胺代谢产物３，４－二羟基苯甲酸和高香草酸含量升高，兴奋性氨基酸及其代谢产物（谷氨酰胺、门冬氨酸和门冬酰胺）含量下降，且３－甲氧－４－羟基扁桃酸、门冬氨酸及门冬酰胺含量与动物行为改变之间具有一定的相关性。结论神经递质代谢紊乱在ＣＳ２的神经毒作用机理中起了重要作用。

Objective In order to study the mechanism of neurotoxic effects of carbon disulfide (CS_2) on animal behavior. Methods Changes in monoamine transmitter, amino acid transmitter and their metabolites in brain tissues were observed in rats exposed to CS_2 at levels of 0,1 200 and 2 400 mg/m^3, respectively, for two months, with high performance liquid chromatography. Results Indicated that exposure to CS_2 could cause decrease in 3-methoxy-4-hydroxy mandelic acid (VMA), increase in 3,4-dioxybenzoic acid, a metabolite of dopamine, and high vanillic acid in rat striatum, decrease in excitatory amino acids and their metabolites (glutamine, aspartic acid and asparagine), with a certain relationship between contents of VMA, aspartic acid and asparagine and changes in neurobehavior. Conclusion Disturbance of neurotransmitter metabolism plays an important role in neurotoxic effects of CS_2.