极度高甘油三脂高胆固醇血症动物模型的建立

Development of hyperlipidemia animal model

建立高脂血症以及联合高脂血症和糖尿病的金黄地鼠模型,为深入研究高脂症在动脉粥样硬化和糖尿病合并动脉粥样硬化等疾病中的作用提供耗时短、效果稳定的动物模型。试验组金黄地鼠(90～110 g)连续3 d腹腔注射小剂量链脲佐菌素(STZ)(30 mg.kg-1)。于注射后9 d,检测空腹血糖,选择血糖大于200 mg.dL-1的个体,给予高脂饲料(HF),对照组同时给予普通饲料(chow)或高脂饲料(HF),检测4周血浆葡萄糖(Glu)、甘油三酯(TG)、总胆固醇(TC)等指标。结果表明,地鼠注射STZ后血糖升高并维持在300～400 mg·dL-1。在喂饲高脂饲料4周后,糖尿病高脂组血脂极度升高,TG达20 000 mg·dL-1,TC达4 500 mg·dL-1;不注射STZ组饲喂高脂饲料后TG和TC也明显升高至1 000 mg·dL-1左右。利用高脂饲喂或联合STZ注射的方法,可以成功制造出稳定的高脂血症以及联合糖尿病的金黄地鼠模型。

The objective of the present study is to develop a quick and stable golden hamster model of hyperlipidemia and a model of diabetes combined with hyperlipidemia, which is suitable for studying the role of hypedipidemia in artherosclerosis. Male hamsters （90-110 g） were divided into two groups and injected intraperitoneally with low dose of streptozotocin （STZ） （30 mg· dL^-1） or vehicle only, 9 d after injection, hamsters which had plasma glucose higher than 200 mg· dL^-1 in STZ group were selected and fed on high-fat diet （HF）, while the control was fed on either HF or chow diet. Plasma glucose（Glu）, triglyeeride（TG）, and total cholesterol （TG） were detected in the following 4 weeks. The results showed that STZ-injected hamsters showed a significant increase in plasma glucose level and maintained at 300-400 mg· dL^-1. TG levels reached 20 000 mg· dL^-1 after 4 weeks treatment with high-fat diet in STZ group, while TC reached 4 500 mg＇dL-1; at the meantime, control group fed on HF also had an increase in plama TG and TC, which reached 1 000 mg· dL^-1. High-fat diet fed hamster or combination of high-fat diet fed and low-dose STZ-treated hamster can be served as an alternative animal model for hyperlipidemia.