灵菊七水提物的降糖作用及促胰岛素分泌作用研究

Effects of aquatic extract of Gynura Medica on anti-hyperglycemia and improving insulin secretion

目的观察灵菊七水提物的降糖作用及对胰岛细胞分泌胰岛素的影响。方法采用四氧嘧啶(70 mg.kg-1)诱导的糖尿病模型小鼠和正常小鼠,连续给药7 d后,观察灵菊七水提物(2.5 mg.kg-1、5 mg.kg-1和10 mg.kg-1)对血糖、血清胰岛素水平和糖耐量的影响;采用M IN6胰岛细胞株,观察灵菊七水提物对胰岛素分泌的影响。结果在给药7 d后,灵菊七水提物可明显降低糖尿病小鼠的血糖水平,并可明显降低正常小鼠和糖尿病小鼠的糖耐量,促进胰岛素的分泌;灵菊七水提物对葡萄糖(16.7 mmol.L-1)刺激下胰岛素的分泌明显具有促进作用。结论灵菊七水提物可通过促进胰岛素分泌来降低血糖。

Aim To investigate the effects of extract of gynura medica on lowering blood glucose and improving insulin secretion. Methods The normal mice and alloxan （70 mg·kg^-1） - induced diabetic mice were administrated for 7 days in order to investigate the effects of aquatic extract of gynura medica on the levels of blood glucose, insulin and oral glucose tolerance. Then, we investigated the effects of the extract on insulin secretion in MIN6 islet cells. Results After 7 days of administration, the extract could significantly lower blood glucose ,which also could ameliorate the oral tolerance in normal mice and diabetic mice and improve insulin secretion. Meanwhile, the extract could improve insulin secretion in MIN6 cells with stimulation of 16.7 mmol ·L^-1 glucose. Conclusion The aquatic extract of gynura medica could lower blood glucose through improving insulin secretion.