水母溶血毒素的生物信息学分析(英文)

Bioinformatic Analysis on Jellyfish Hematoxin

目的与方法:为阐明已报道的5种水母溶血毒素的氨基酸组成和序列、信号肽、跨膜结构域、疏水性/亲水性、二级结构、保守区域、分子进化关系等,利用生物信息学方法对其进行了分析和预测。结果与结论:不同水母溶血毒素的氨基酸组成和理化性质相类似;水母溶血毒素存在跨膜结构域和疏水区域;在20～21位点最有可能存在信号肽切割位点;α螺旋、不规则卷曲是二级结构中最大量的结构元件,β折叠散布于整个蛋白质中;在其中3种水母毒素蛋白中存在4个保守区;以MP法和NJ法构建的系统发生树基本一致。

AIM： To illustrate the structure and function of five hematoxin sequences reported in four jellyfish and explore the possible mechanism of pathogenesis. METHODS： Bioinformatic methods were used to analyze the composition and sequence of amino acid residues, physico-chemical property, signal peptide, membrane spanning domain, hydrophobicity or hydrophilicity, secondary structure, conserved region and molecular phylogenetic evolution. RESULT and CONCLUSIONS： The results showed that the five jellyfish hematoxins were similar in composition and sequence of amino acid residues and physico-chemicai property. The amino acid sequences of jellyfish hematoxins contained membrane spanning domain and hydrophobic regions; with a possible cleavage site in the signal peptide between the amino acid residues 20 and 21; a-helix and random coil were the major motifs of predicted secondary structure while ,B-turn and extended strand spread in the whole protein; There were four conserved amino acid sequences in three of the five hematoxins and similar phylogenetic trees were constructed by both NJ and MP methods.