碱性双氧水法预处理木质纤维素

Pretreatment Process for Lignocellulose by Alkaline H_2O_2

在采用木质纤维素制备燃料乙醇工艺中,纤维素酶对纤维素物质的可及性对纤维素的酶解过程非常重要,木质纤维素物质的预处理过程就是通过溶解木质素、半纤维素以及破坏纤维素的结晶结构,以提高纤维素酶的可及性。采用碱性双氧水法处理木质纤维素,应用部分重复因子设计的实验方法考察了反应时间(tR)、反应温度(Te)、双氧水用量(ch)、液固比(Vr)和NaOH浓度(cb)5个因素对预处理效果的影响,确定tR=18 h、Te=70℃c、h=1%、Vr=8 g.L-1、cb=10 g.L-1为较优化的条件。

It is very important for reachability of cellulase to cellulose in the process of fuel ethanol production from lignocellulose. The purpose of pretreatment of lignocellulose before cellulase hydrolyzation is to improve cellulase＇s reachability by dissolving lignin, hemicellulose and breaking the crystal structure of cellulose. Pretreatment process for lignocellulose by alkaline H2O2 was introduced in this article. In the same time, five factors including reaction time （tR）, reaction temperature （Te）, H2O2amount （Ch）, the ratio between liquid and solid （Vr）, and concentration of NaOH （Cb） were researched by fractional factorial design. Finally, the relatively optimized condition of tR=18 h,Te=70℃ ,Ch=1% ,Vr=8 g · L^-1 and Cb=10 g · L^-1 was acquired.