腐竹废水制备大豆多肽复合酶解条件研究

Study on Composite Enzymatic Hydrolysis Technology for Soybean Peptide from Yuba Wastewater

将腐竹废水进行回收利用,经蛋白酶酶解、分离、提纯制得大豆多肽。通过正交实验分析,确定腐竹废水复合酶解的最佳工艺条件为:采用三酶复合蛋白酶(由碱性蛋白酶、木瓜蛋白酶和菠萝蛋白酶以1∶2∶4的比例复合而成)、底物含量3%、pH值8.0、酶解时间4 h,此时多肽转化率为94.56%。

After the protease hydrolysis, separation and purification, the soybean peptide was obtained from yuba wastewater. Through orthogonal test analysis, the optimum composite enzymatic hydrolysis conditions were determined as follows：the complex enzyme was made by alkaline protease, papaya protease and pineapple protease with a ratio of 1 ： 2 ： 4, the substrate content was 3%, pH value was 8.0 and hydrolysis time was 4 h. Above these conditions the peptide enzyme conversion could reach 94.56%.