光谱法研究卟啉锌(HP—Zn)配合物在超声波作用下对牛血清白蛋白(BSA)的损伤

Study on damage of BSA in the presence of HP—Zn complex under ultrasonic irradiation by spectrophtomerty

应用荧光光谱和紫外-可见光谱研究了卟啉锌（HP—Zn）与牛血清白蛋白（BSA）的相互作用及在超声波作用下HP—Zn对BSA的损伤作用，并探讨了超声照射时间，HP—Zn浓度和溶液酸度等因素对BSA损伤的影响。结果表明，37℃时，BSA与HP—Zn的结合数n=1．0379，其平衡常数KA=6．2661×10^4，两者之间的作用距离r=3．53nm。在一定条件下，BSA的损伤程度随着超声照射时间．HP—Zn浓度和溶液酸度的增大而增大。这一结果对声动力学疗法（SDT）治疗肿瘤应用于临床具有重要的意义。

The interaction between hematopotphyrin zinc （HP-Zn） and bovine serum albumin （BSA） and damage of BSA in the presence of HP-Zn under ultrasonic irradiation were studied by UV -Vis and fluorescence spectra. The influences of ultrasonic irradiation time, HP-Zn concentration and solution acidity on damage of BSA were also detected. The results showed that at 37℃, the binding site number （n）, the apparent binding constant （KA） and the binding distance （r） were 1. 0379, 6. 2661 × 10^4and 3.53 nm, respectively. Under different conditions, the damage degree of BSA rose up with the increasing of ultrasonic irradiation time, HP-Zn concentration and solution pH value. The research results are of great significance for driving sonodynamic treatment （SDT） to clinic application.