顺行穿刺注射牛磺胆酸钠制作大鼠重症胰腺炎模型

Severe acute pancreatitis model by antegrade puncture injection of the sodium taurocholate in rat

目的探讨制备大鼠重症胰腺炎模型方法的改进。方法将36只健康sD大鼠随机分为模型组（n=18）和假手术组（n=18），在胰胆管的近端（肝总管）用细针顺行穿刺胰胆管，注入4％牛磺胆酸钠，诱导重症急性胰腺炎动物模型，创面封闭胶封闭穿刺孔；两组均在模型诱导3、6、12h后检测腹水量及血淀粉酶，观察胰腺组织形态变化，光镜下行胰腺病变程度评分。结果模型组在模型诱导后腹水量（8．52±1．05）ml、血淀粉酶（5247．17±547．07）u／L、胰腺组织学评分（13．60±3．92）较假手术组腹水量（1．21±0．32）ml、血淀粉酶（1289．5±176．67）u／L、胰腺组织学评分（0．83±0．58）在各时间点均明显升高（P〈0．05）。结论此法损伤小，实验器材易于获取，操作简单，不会损伤胰腺和发生胆漏，可重复性好，模型稳定，成功率高，是较理想的重症胰腺炎模型制备方法。

Objective To improve the establishment of severe acute pancreatitis model in rat. Methods 36 Sprague-Dawley rats were randomly divided into experiment or shame operation group（ n = 18, each）. Severe acute pancreatitis was induced by injection of 4% sodium taurocholate through puncturing common bile duct with a fine needle, puncture hole was closed by medical sealant glue. Ascites volume and blood amylase were meas- ured at 3, 6 and 12 hours after injection. Gross and histological changes of pancreas were evaluated by a scoring system. Results Pancreatic changes in the experiment group was hemorrhagic and necrosis. The ascites volume （ 8.52 ± 1.05 ） ml, serum amylase activity （ 5247.17 ± 547.07 ） u/L, gross and histological scores （ 13.6± 3.92 ） in experiment group was significantly higher than ascites volume（ 1.21 ± 0.32 ）ml, serum amylase activity（ 1289. 5 ± 176.67）u/L, gross and histological scores（ （0.83±0.58） in shame operation group at every time point（P 〈0. 05）. Conclusions Combined injection through common bile duct and medical sealant glue in the experiment can minimize the trauma and simplize the procedure. This method produces a reliable model with high success rate and it is an ideal severe acute pancreatitis animal model.