一种基于介质上电润湿效应的免疫检测芯片研究

An Immunoassay Chip Based on Electrowetting on Dielectrics

利用微机械加工技术,在ITO玻璃上设计制作了基于介质上电润湿效应的以离散液滴为对象的免疫检测芯片,对芯片的液滴驱动特性、免疫反应参数进行了测试,并对小鼠IgG和羊抗鼠IgG-HRP进行了初步的免疫测试。研究结果表明,在电压<100V的时候,接触角测量值基本上和预测曲线吻合,在>100V的情况下出现了接触角饱和现象,在芯片上实现了液滴操纵,120V时得到最大平均速度为3.75mm/s;该芯片可以实现免疫反应检测,所需样品体积为0.5μL,检测时间约为20min,对于羊抗鼠IgG-HRP实验系统的检测范围为0.1～20mg/L。

An immunoassay chip based on electrowetting on dielectrics（EWOD） droplet control method was developed. Basing on the process of immunoassay, the immunoassay chip was designed, and fabricated using indium-tin-oxides（ITO） coated glass as substrate. We tested the controllability performance on droplet actua- ting of the chip. The experimental results of contact angles of the droplet were closer to the theoretical predictions within 100 V. The largest speed of the droplet movement was up to 3.75mm/s, and the droplet manipulations were achieved by EWOD on the ITO-coated glasses. By the immunoreaction of rat IgG and goat anti-rat IgG on the immunoassay chip, the possibility of the immunoreaction on the immunoassay chip was validated. The volume of sample is 0.5μL and the detected time is 20 min. The detected range of the immunoreaction of rat IgG and goat anti-rat IgG is 0.1 -20 mg/L.