CD31与CD34显示非小细胞肺癌微血管密度的对比分析

Comparison of CD31 and CD34 in showing microvessel density in non-small cell lung cancer

目的:探讨血管内皮标志物CD31和CD34在显示非小细胞肺癌(NSCLC)微血管密度(MVD)中的差异以及与临床病理特征的关系。方法:采用免疫组化染色方法和Weidner计数标准检测CD31和CD34在57例NSCLC组织中的表达。结果:CD31和CD34显示的MVD结果分别为(29.7±12.1)条和(38.2±12.7)条,范围分别是10～61条和19～76条,两者差异有统计学意义(P<0.001),但相关性良好(P<0.05);不同临床病理特征的MVD计数均无统计学意义(P>0.05)。结论:同用ElivisionTM plus法,CD34染色效果优于CD31,更适合于临床应用;CD34和CD31 MVD计数显示其结果与各临床病理特征无明显相关性。

Objective ：To study the difference between CD31 and CD34 in showing the microvessel density（MVD） of non-small lung cancer（NSCLC）, and to investigate the relationship between MVD and clincopathological parameters. Methods： ElivisionTM plus immunohistochemical techinique was used to detect the expression of CD31 and CD34 in 57 cases of NSCLC. Results：The MVDs shown by CD31 and CD34 were （29.7 ± 12.1 ） and （38.2 ± 12.7） respectively,within a range of 10 - 61 and 19 - 76. The difference was signifieant（P 〈 0. 001 ）, hut the correlation was good （P 〈 0.05 ）. No significant difference between MVD and elinopathological parameters was observed（P 〉 0.05）. Conclusions：The endothelial cell of micro-vessels can be shown by both CD31 and CD34,but CD34 is better than CD31 and is more suitable for clinical application. There is no significant difference between MVD and clinopathological parameters including metastasis of lymph node.