纤维蛋白原β基因启动子-845G/A多态性与早发冠心病相关性

Relationship between fibrinogen-β-845G/A polymorphism and premature coronary heart disease

目的:探讨纤维蛋白原β(Fg-β)基因启动子-845G/A多态性与中国早发冠心病(CHD)发病的相关性。方法:早发CHD组78例,对照组81例,采用苯酚-氯仿法提取DNA,聚合酶链反应-限制性片段长度多态性方法(PCR-RFLP)分析Fg-β基因启动子-845G/A多态性,收集临床资料,检测血糖和血脂等生化指标。结果:早发CHD组与对照组Fg-β基因启动子-845G/AA、GG、GA基因频率及A、G等位基因频率差异均无统计学意义(P>0.05);但两组间总胆固醇、低密度脂蛋白、载脂蛋白B、脂蛋白a均有统计学意义(P<0.05～P<0.01)。结论:Fg-β基因启动子-845G/A多态性可能与中国早发CHD的易感性无明显关系。

Objective： To investigate the relationship between fibrinogen-β-845A/G polymorphism and premature coronary heart disease. Methods： Seventy-eight cases of premature coronary heart disease and 81 controls were enrolled in the study. Phenoltrichlormethane was employed to extract DNA, polymerase chain reaction-restriction fragment length polymorphism （PCR-RELP）was used to analyze Fg-β gene polymorphism, and the clinical data were collected. The blood sugar, blood lipids and other biological indicators were tested. Results ：There was no significant difference in genotype distribution and alleles of fibrinogen-β-845A/G between patients with premature coronary heart disease and controls （ P 〉 0.05 ）, but the difference was significant in total cholesterol, lowdensity lipoprotein-C ,apolipoprotein B and lipoprotein-a between the two groups （P 〈 0.05 - P 〈 0.01 ）. Conclusions：The results suggest that fibrinogen-β-845A/G poly-morphism may not be associated with the susceptivity of premature coronary heart disease in China.