高效液相色谱法同时测定人血浆中齐多夫定、奈韦拉平浓度

Simultaneous Determination of Zidovudine and Nevirapine in Human Plasma by HPLC-UV

目的高效液相色谱法同时测定齐多夫定和奈韦拉平血浆中的浓度。方法色谱柱为岛津Shim-pack CLC-ODS(6mm×15cm,5μm)。流动相为乙腈-水(23∶77),流速1mL·min-1。紫外检测波长为260nm。柱温为室温。内标物(替加氟)与齐多夫定、奈韦拉平的保留时间分别为4.665,5.198和10.912min。结果齐多夫定、奈韦拉平的线性范围分别为0.025～10mg·L-1(r=0.9999)和0.05～10mg·L-1(r=0.9999),最低检测下限分别为0.025和0.05mg·L-1。样品溶液冻融稳定,日内精密度和日间精密度良好,回收率较高。结论采用HPLC-UV测定血浆中齐多夫定和奈韦拉平浓度,方法简便,结果准确可靠。

OBJECTIVE To establish a method for simultaneous determination of zidovudine（AZT） and nevirapine（NVP） in human plasma. METHODS The concentration of zidovudine and nevirapine were analyzed on a Shim-pack CLC-ODS （6 mm× 15 cm, 5 μm） with a mobile phase consisting ofwater-acetonitrile （23 ： 77） at a flow rate of 1 mL·min^-1. The wavelength was 260 nm. The retention time of internal standard（tegafur）, zidovudine and nevirapine were 4.665, 5.198 and 10.912 min, respectively. RESULTS The calibration curves of AZT and NVP were linear with the range of 0.025-10 mg·L^-1 （r=0.999 9） and 0.05-10 mg·L^-1 （r=0.999 9）, and the limits of detection were 0.025 and 0.05 mg·L^-1, respectively. The average recoveries of AZT and NVP were both good. CONCLUSION The method was simple, practical and reliable to be used for the study of pharmacokinetics and pharmacodynamics.