马尾藻多糖的抗肿瘤作用及其免疫功能的研究

Antitumor activity and immune activity of polysaccharides sargassum

分别以马尾藻多糖(SP)、环磷酰胺对H22荷瘤NIH小鼠灌胃给药,通过建立H22实体瘤小鼠模型和H22腹水瘤小鼠模型,测定其抑瘤率、胸腺指数、脾指数、免疫球蛋白及T淋巴细胞数量等指标,观察马尾藻多糖在H22腹水瘤小鼠体内的抗肿瘤作用及其机理、对H22腹水瘤小鼠生命延长的影响和体内T淋巴细胞数量的变化。结果表明,马尾藻多糖对H22荷瘤小鼠有明显的抑瘤作用,其质量比为100 mg/kg时的抑瘤效果最好,抑瘤率达到61.3%;H22腹水瘤小鼠经SP灌胃后,胸腺指数、脾指数、T淋巴细胞数和白细胞数均有明显升高;SP能显著提高H22腹水瘤小鼠的平均生存时间,SP质量比为100 mg/kg时效果最好,H22腹水瘤小鼠生命延长率为69.87%。

This paper deals with the systemic effects of polysaccharides Sargassum （SP） on NIH mice with H22 tumor. The antitumor effort and immune function of SP were evaluated. H22 tumor bearing mice entity model was established, was treated by gavage with different does of SP and the inhibition rates, thymus and spleen index T lymphocytes and WBC were measured, the vivo deflects on the antitumor were studied. H22 tumor bearing ascites mice model was established and the average survival time was measured. The SP have an evidence in vivo antitumor effect. The inhibition rates are 61.3 % on H22 in the concentration of 100 mg/kg. Treatment by gavage with SP can increase the thymus and spleen index, strengthen T lymphocytes and WBC and increase the IgA, IgM, IgG. The SP can improve the average survival time on the ascites mice, the highest inhibition rate is 69.87% in the concentration of 100 mg/kg. The SP has a definite antineoplastic effect and promotes the immune function of tumor bearing mice.