知母中芒果苷的超声提取工艺研究

Study on ultrasonic extraction technics of mangiferin in Anemarrhena asphodeloides Bge

研究超声波辅助提取法从知母中提取芒果苷的最佳工艺条件。采用单因素及正交实验,考察了乙醇浓度、超声时间、超声功率及固液比对芒果苷提取量的影响规律,用HPLC测定芒果苷含量。芒果苷提取工艺的最佳参数组合为乙醇体积分数60%、超声时间30min、超声功率为40W、固液比为1∶70(g/mL),在最佳参数组合下芒果苷的得率可达1.85%。以此方法提取,具有提取时间短、简单、收率高、无需加热等优点。

To study the best extraction condition of ultrasonic extraction of mangifedn in Anemarrhena asphodeloides Bge. The influencing rules of the mangiferin in Anemarrhena asphodeloides Bge were studied basing on the yield of extracts. Orthogonal experiment was carried out to investigate 4 influential factors of alcohol concentration, ultrasonic extracting time, ultrasonic power and ratio of solid to liquid. HPLC was used for the determination. The best extraction condition of mangiferin in Anemarrhena asphodeloides Bge were determined, which showed that alcohol concentration was 60%, ultrasonic extracting time was 30 min, ultrasonic power was 40 W and ratio of solid to liquid was 1.70（g/m L）. In this extraction condition, the yield of extracts of mangiferin in Anemarrhena asphodeloides Bge was 1.85%. Ultrasonic method can save time, be easy to operate, improve extraction rates and need no heating.