日粮能量水平对猪脂肪酸合成酶和激素敏感酯酶的影响

Effect of Dietary Energy Levels on the Activity and mRNA Expression of Fatty Acid Synthase and Hormone-Sensitive Lipase in Pigs

选择初始体重约20kg的长白×荣昌杂交猪72头,随机分为3个处理,研究日粮能量水平对生长育肥猪脂肪酸合成酶(FAS)和激素敏感酯酶(HSL)活性及基因表达量的影响。日粮消化能分别为11.75MJ/kg(低能量组)、13.05MJ/kg(中能量组)和14.36MJ/kg(高能量组)。试验猪体重达到100kg时屠宰。结果表明:与低能量组相比,中能量组和高能量组的脂肪组织、肝脏组织和肌肉组织的FAS活性显著提高(P<0.05),而HSL活性明显降低,并且FAS和HSL活性具有组织差异性,脂肪组织中活性最高,肝脏组织中的活性其次,肌肉组织中的活性最低;与低能量组相比,中能量组和高能量组的脂肪组织和肌肉组织的FASmRNA表达量均明显提高,HSLmRNA表达量均显著降低(P<0.05)。本研究结果初步表明,能量水平调控脂肪沉积可能是通过调节FAS和HSL的活性及基因表达量来实现的。

A total of 72 Danish Landrace x Rongchang crossbred pigs with average weight of 20 kg were randomly allocated into 3 treatments to investigate the effect of energy level on the activities and gene expressions of fatty acid synthase （FAS） and hormone-sensitive lipasw （HSL）. The digestible energy levels of diets were 11.75, 13.05 MJ/kg and 14.36MJ/kg, respectively. The pigs were slaughtered at 100 kg body weight. The result showed that Compared with lowenergy density group, the activities of FAS in fat tissue, liver and muscle for middle-energy density group and high- energy density group were significantly increased （P 〈 0.05）; and the activities of HSL were obviously decreased. There was tissue difference for the activities of FAS and HSL The activities of FAS and HSL in fat tissue were higher than those in liver and muscle.Compared with low-energy density group, the FAS mRNA expressions in fat tissue and muscle for middle-energy density group and high-energy density group were obviously increased, and the expressions of HSL mRNA in fat tissue and muscle were significantly decreased with energy density increasing （P 〈 0.05）. It was concluded based on the above results that dietary energy level could influence fat deposition in pigs associated with the regulation on the activities and gene expressions of FAS and HSL.