鱼油与硒对饲喂亚麻籽日粮绵羊体组织脂肪酸组成及抗氧化的影响

Effects of Fish Oil and Selenium on Tissues Fatty Acid Composition and Antioxidant Status of Sheep Fed the Linseed Diet

研究0.33%鱼油与2mg/kgDM的有机硒对饲喂亚麻籽日粮的绵羊体组织脂肪酸组成及抗氧化功能的影响。初步探讨在绵羊日粮中添加亚麻籽提高体组织CLA含量的同时,再添加鱼油和硒是否能进一步提高CLA含量。选用体重相近,健康的杂交一代公羔羊16只,随机分为4组。4组日粮为对照组LD组(基础日粮+10%亚麻籽),LF组(基础日粮+0.33%鱼油+9.17%亚麻籽,其中0.33%鱼油+9.17%亚麻籽所含脂肪与10%亚麻籽脂肪含量相等),LDSe组(基础日粮+10%亚麻籽+2mg Se/kgDM);LFSe组(基础日粮+0.33%鱼油+9.17%亚麻籽+2mgSe/kg DM),硒以有机硒的形式添加。结果表明:硒的添加对绵羊血浆抗氧化功能无显著影响(P<0.05),可能是受基础日粮硒浓度的影响;亚麻籽日粮中添加0.33%鱼油提高了绵羊体组织cis-9,trans-11-CLA和trans-11-C18:1含量。亚麻籽日粮中添加2mg Se/kg DM的有机硒对绵羊体组织脂肪酸组成没有显著影响。鱼油与有机硒的添加对绵羊不同组织△9-脱氢酶活性各组间影响差异不显著。由此表明,日粮中联合添加亚麻籽+鱼油以提高组织CLA含量的效果优于单独添加亚麻籽。

The experiments were conducted to study the effects of 0.33% fish oil and Se 2 mg/kg DM （as organic selenium） on the fatty acid composition of tissue in sheep fed the linseed diet, also on the antioxidant status in plasma. A total of 16 male lambs with similar body weight were divided randomly into four groups and each was fed one of the following diets, LD group （basal diet ＋ 10% linseed）, L F group （basal diet ＋ 9.17% linseed ＋ 0.33% fish oil. Fish oil was proportionately 0, 0.33 of the oil supplement, with linseed providing the balance of added fat）. LDSe （basal diet ＋ 10% linseed ＋ Se 2 mg/kg DM） LFse group basal diet ＋ 9.17% linseed ＋ 0.33% fish oil ＋ Se 2 mg/kg DM（The Se was added as organic selenium）. The experiment lasted 60 days and lambs were slaughtered at the end of it. The results showed that 0.33% fish oil and 2 mg Se/kg DM added to the linseed diets had no significant effect on the antioxidant status in plasma due to the effect of selenium concentration of basal diet; 0.33%fish oil added to the linseed diets could increas the concentration of cis 9, trans- 11 -CLA and trans-11-C18：1 in tissues. Se 2 mg/kg DM added to the linseed diets had no effect on the fatty acid composition in tissues 0.33% fish oil and Se 2 mg/kg DM added to the linseed diets had no significant effect on the A9-desaturase activity in tissues. It showed that the approach to increase CLA content in tissues was by combining fish oil and linseed, which was better than adding linseed alone.