咪唑克生对实验性自身免疫性脑脊髓炎大鼠脊髓内胶质细胞变化的影响

Effects of idazoxan on changes of neuroglia in spinal cords of rats with experimental autoimmune encephalomyelitis

目的:探讨咪唑克生(Idazoxan,Ida)对实验性自身免疫性脑脊髓炎(Experimental autoimmune encephalomyelitis,EAE)大鼠脊髓内神经胶质细胞变化的影响及其意义。方法:采用临床症状评分、病理学检查和免疫组化观察咪唑克生处理条件下豚鼠全脊髓匀浆诱导的Lewis大鼠EAE临床表现、病理改变及脊髓内星形胶质细胞和小胶质细胞变化。结果:咪唑克生虽不减低Lewis大鼠EAE的发病率,但可减轻其临床症状和病理学改变。在免疫后15天,炎性脱髓鞘病灶内和周围星形胶质细胞数量增多,胞体肥大,与此相反,小胶质细胞数量是减少的。结论:Ida对实验性自身免疫性脑脊髓炎大鼠具有保护作用,其作用机制可能为咪唑克生对CNS的免疫机制有一定影响。

Objective：To explore effects of Idazoxan（IDA） on changes of neuroglial cells in spinal cords of rats with experimental autoimmune encephalomyelitis（EAE）.Methods：Rat EAE was induced by immunization with spinal cord homogenates of Geania pigs.EAE clinical manifestations were assessed in terms of the scoring standards.Histological investigation and immunohistochemistry were observed for the inflammatory dedmylinative lesion of CNS and morphology of glial cells.Results：Ida could not decrease the incidence of EAE,but alleviate its clinical manifestation and histological changes.On day 15 after immunization,astrocytes in and around the inflammatory dedmylinative lesion of CNS in EAE rats treated with Ida increased in number and size,on the contrary,microglia decreased in number and size.Conclusion：Ida has protective effects on EAE and its functional mechanism may be concerned with modulation of immunological mechanism of CNS.