前体饲喂和真菌诱导子对肉苁蓉悬浮培养细胞次生代谢产物的影响

Effects of Precursor Feeding and Fungal Elicitors on Secondary Metabolits in Cell Suspension Culture of Cistanche deserticola

目的:研究前体饲喂和真菌诱导子对肉苁蓉悬浮培养细胞生长、苯乙醇总苷(PeGs)和松果菊苷(Ech in)含量的影响。方法:悬浮培养联合加入前体和真菌诱导子。结果:前体化合物L-苯丙氨酸+L-酪氨酸与番茄霉菌联合作用,肉苁蓉悬浮细胞干重、PeGs及Ech in含量均达到最大值,分别为14.69 g DW/L、50.55 mg/g和23.86mg/g,是对照的1.81、4.18和3.99倍。结论:前体和诱导子联合作用能显著提高肉苁蓉细胞悬浮培养体系中次生代谢物的含量。

Objective： To investigate the effects of precursor feeding and fungal elicitors on cell growth and the contents of PeGs and Echin in the cell supension culture of Cistanche deserticola. Methods： The combination of precursor feeding and fungal elicitors were added on the cell suspension culture. Results： The combination of L-phenylalanine and L-Tyrosing were added with Cladosporiurn. fulvum, the dry weight of cell and the contents of PeGs and Echin in suspention all reached the highest value, 14. 69 gDW/L, 50. 55 mg/g and 23.86 mg/g, which were 1.81,4. 18 and 3.99 times as much as the values of controls, respectively. Conclusions： Precursor feeding and fungal elicitors had obvious promotion effects on cell growth and contents of PeGs and Echin in cell suspension culture of C. deserticola.