石榴皮提取物对大鼠实验性前列腺炎的影响

Effect of Pomegranate Peel Extracts on Experimental Prostatitis Rats

目的:探讨石榴皮提取物(Pom egranate peel extracts,PPE)对大鼠非细菌性前列腺炎(NBP)和细菌性前列腺炎(CBP)的抑制作用,并通过观察其对大鼠血清酸性磷酸酶及前列腺组织中锌(Zn)含量的影响,分析其可能的作用机制。方法:给药7天后分别向SD大鼠前列腺内注射角叉菜胶和金黄色葡萄球菌制备大鼠非细菌性前列腺炎和细菌性前列腺炎模型,术后2天检测大鼠前列腺组织卵磷脂小体的密度、白细胞个数、血浆酸性磷酸酶(ACP)活性,前列腺组织匀浆液中锌含量、丙二醛水平(MDA)、抗氧化能力指数及血浆一氧化氮(NO)的含量。结果:石榴皮提取物可显著提高前列腺组织中锌含量、卵磷脂小体密度,降低前列腺组织匀浆液中白细胞个数及血浆酸性磷酸酶活性,并且能提高血浆的抗氧化能力指数。结论:石榴皮提取物对大鼠非细菌性前列腺炎和细菌性前列腺炎的抑制作用可能与改善酸性磷酸酶活性、前列腺组织Zn含量及机体的氧化应激状态相关。

Objective：To investigate the inhibitory effects of Pomegranate peel extracts （PPE）on experimental prostatitis rats. Methods：The Prostatitis model was established by injection of carrageenan and staphylococcus aureus in rat prostate. After 9 days＇ treatments of PPE,the rats were sacrificed for measurement of the number of white blood cell（WBC）, the density of lecithin, the levels of Zn and malondialdehyde （MDA） of prostate. The antioxidant capacity was measured by oxygen radical absorbance capacity （ORAC） assay. The levels of NO and the activity of acid phosphatase （ACP） of plasma were also measured. Results：PPE increased the levels of ORAC in plasma and the density of lecithin and the levels of Zn, decreased the levels of MDA of prostate and the activity of ACP and the number of WBC and adjusted the levels of NO in plasma compared with the prostatitis model group （P 〈0. 05 or P 〈 0. 01 ）. Conclusion： PPE can markedly improve the protective function of oxidation resistance, it may involve its regulatory action on anti-oxidation.