石菖蒲挥发油对心肌细胞形态学及细胞活力的影响

Effects of Volatile Oil of Rhizoma Acori Tatarinowii on Morphology and Cell Viability in Cultured Cardiac Myocytes

目的：观察石菖蒲挥发油对正常心肌细胞形态学及细胞活力的影响。方法：采用胰酶冷消化法消化培养心肌细胞，用免疫细胞化学方法鉴定心肌细胞纯度；将不同浓度石菖蒲挥发油与心肌细胞作用24h，用倒置显微镜观察细胞形态的变化，并用MTT比色法检测细胞活力。结果：原代培养的乳鼠心肌细胞纯度达95％以上；不同浓度的石菖蒲挥发油能降低正常心肌细胞的搏动频率；终浓度为100—160mg／L的石菖蒲挥发油能提高心肌细胞活力D（λ）值，与基质组比较有统计学差异，其量效关系是以140mg／L剂量组D（λ）值为顶点的抛物线。结论：一定浓度的石菖蒲挥发油有降低心肌细胞搏动频率、提高心肌细胞活力的作用。

Objective ：To observe the effects of volatile oil of Rhizoma Acori Tatarinowii （VOSCP） on morphology and cell viability in cultured neonate rat cardiac myocytes. Methods：The cardiac myocytes were cultured by trypsin under cool treatment, and the cell purity was assayed with imminocytochemistry;Morphological changes were observed under phase contrast microscope after cardiac myocytes with VOSCP of different concentration for 24h in vitro, and the cell viability was examed by MTT assay. Results：The purity of cultured neonate rat cardiac myocytes was higher than 95% ;VOSCP of different concentration could depress pulse frequency and 100—160 mg/L VOSCP can obviously improve the viability of cardiac myocytes. The relation of dose-effect relationship was a parabola whose peak was at the value of 140 mg/L. Conclusion：VOSCP of proper concentration can depress pulse frequency and improve the viability of cardiac myocytes.