黑曲霉Aspergillus niger S菌株所产β-葡糖苷酶的纯化和酶学性质

Purification and characterization of β-glucosidase from Aspergillus niger

利用黑曲霉Aspergillus niger S菌株,通过液体发酵的方法,得到了一种可同时高效水解大豆异黄酮糖苷成大豆异黄酮,水解白藜芦醇苷成白藜芦醇的糖基水解酶.该酶经硫酸铵分级沉淀、离子交换层析及分子排阻色谱纯化得单一条带,经SDS聚丙烯酰胺凝胶电泳测得该酶的表观分子量约为165ku①,比通常所报道的传统β-葡糖苷酶都大,该酶的最适反应温度为50℃;最适pH值为5～10.

strain. The A high activity glucosidase, which can hydrolyze glycosides, was isolated from AspergiUus niger S enzyme was purified to one spot after ammonium sulfate precipitation, ion exchange ＆ size exclusion chromatography and its molecular weight was about 165ku, which was quite differ from other reported glucosidase. The optimum temperature of this enzyme was 50℃ ＆ the optimum pH was pH 5 - 10. Based on the HPLC result, this enzyme can transform glycone to aglycone of isoflavones ＆ resveratrol with high specific activity.