慢性前列腺炎患者前列腺液细菌16S rRNA基因的检测

Detection of 16S ribosomal RNA gene of bacteria in prostatic secretion of patients with chronic prostatitis

目的通过采用聚合酶链反应(PCR)技术对慢性前列腺炎患者前列腺液中细菌16S rRNA基因检测,探讨PCR方法诊断慢性前列腺炎细菌学病因的价值。方法收集102例慢性前列腺炎患者的前列腺液进行细菌培养,同时采用PCR法检测前列腺液中细菌16S rRNA基因,并测定和分析所得片段的DNA序列。对培养结果与PCR法检测结果进行了比较。结果102份前列腺液标本中,细菌培养法18例阳性,84例阴性,阳性率17.7%;PCR法71例阳性,31例阴性,阳性率69.6%。细菌培养法与PCR法检测结果比较,其差异有统计学意义(P<0.05)。测序结果显示DNA序列与GenBank公布的16S rRNA基因序列具有很高的同源性。结论PCR技术对慢性前列腺炎的临床诊断具有重要意义。

Objective To explore the role of bacteria in chronic prostatitis by detecting the bacterial 16S ribosomal RNA gene of the expressed prostatic secretion （EPS） using polymerase chain reaction （PCR）. Methods EPS from 102 patients with chronic prostatitis were performed bacteria culture and analyzed by PCR for bacterial 16S ribosomal RNA gene. The DNA fragments were directly sequenced and compared with the 16S ribosomal RNA sequences reported in GenBank. At the same time, the results of two methods were compared. Results Among 102 EPS specimens, there were 18 positive and 84 negative analyzed by culture, and the positive rate of bacteria culture wasl7.7%. There were 71 positive in 16S ribosomal RNA gene and 31 negative analyzed by PCR, and the positive rate was 69.6%. There were sig- nificant difference between the result of bacteria culture and PCR （P 〈 O. 05 ）. The DNA sequences had significantly ho- mology with 16S ribosomal RNA gene. Conclusions PCR technique plays an important part in clinical diagnosis of chronic prostatitis.