利用电子自旋共振(ESR)技术对秘鲁鱿鱼中甲醛生成非酶途径中相关自由基的研究

Studies on the Non-enzymic Formation Radical of Formaldehyde in Jumbo Squid by ESR Method

利用电子自旋共振仪(ESR)测定秘鲁鱿鱼中与甲醛生成相关的自由基,结果显示,在秘鲁鱿鱼加热过程中伴随着甲醛的生成,产生了(CH3)3N·自由基。为进一步证明(CH3)3N·自由基与甲醛生成的相关性,在TMAO-Fe(II)体系中研究(CH3)3N·自由基的生成规律。(CH3)3N·自由基随温度的上升而增多,与甲醛生成规律一致。(CH3)3N·产生后,在室温条件下,10min内信号强度迅速衰减,随后缓慢衰减。因此初步证明秘鲁鱿鱼中甲醛产生的非酶途径中存在自由基反应。

The radical which is relate to formaldehyde in jumbo squid was determinated by ESR. The results showed that （CH3）3N· radical was produced when formaldehyde formed in jumbo squid during heating treatment： For further identification of the relationship between（CH3）3N· radical and formaldehyde formation, the formation regulars of （CH3）3N· radical was studied in TMAO-Fe （II） system. Among of them, the formation of （CH3）3N· radical was increased with the temperature increased which is according to reqularity of formaldehyde. The signal intensity of （CH3）3N· radical was decreased quickly in 10 min, and then decreased slowly. This paper indicated that radical reaction was existed in non-enzymic formation mechanism of formaldehyde in jumbo squid.