API_(0134)对LDL诱导单核细胞表达PDGF-B、bFGF及平滑肌细胞增殖的抑制作用

Inhibitory effects of API_(0134) on PDGF-B and bFGF expression in monocyte induced by slight-oxidative modified low density lipoprotein

目的和方法：应用免疫细胞化学方法，观察穿心莲成分ＡＰＩ０１３４对轻度氧化低密度脂蛋白（ｍｍＬＤＬ）诱导人血单核细胞表达血小板源生长因子Ｂ链蛋白（ＰＤＧＦ－Ｂ）和碱性成纤维细胞生长因子（ｂＦＧＦ）的影响；并进行平滑肌细胞（ＳＭＣ）有丝分裂试验，观察ＡＰＩ０１３４作用后的单核细胞条件培养基（ＣＭ）对３Ｈ－ＴｄＲ掺入ＳＭＣＤＮＡ的影响。结果：ｍｍＬＤＬ可显著促进单核细胞表达ＰＤＧＦ－Ｂ和ｂＦＧＦ，ｍｍＬＤＬ作用后的ＣＭ亦可显著增加３Ｈ－ＴｄＲ的掺入量。ｍｍＬＤＬ的上述作用可被不同剂量的ＡＰＩ０１３４和ＡＰＩ０１３４作用后的ＣＭ所抑制。结论：ＡＰＩ０１３４的作用可能是抗动脉粥样硬化的机理之一。

AIM and METHODS:The effects of API0134 on expression of platelet-derived growth factor B chain protein(PDGF-B) and basic fibroblast growth factor(bFGF) in human monocytes induced by slight-oxidative modified low density lipoprotein(mmLDL) were studied by immunocytochemical methods,and the effects of monocyte conditioned media(CM) on 3H-TdR in corporation into DNA of smooth muscle cell(SMC) were observed. RESULTS: mmLDL induce the expression of PDGF-B and bFGF in monocytes significantly, and 3H-TdR incorporation into DNA of the SMC were also promoted significantly by the CM treated with mmLDL. The inductive effect of mmLDL can be inhibited by API0134 as well as by CM treated with API0134. CONCLUSION:The inhibitory effects of API0134 may be one of its anti-atherosclerotic mechanisms..