眼镜蛇毒直接溶解因子对人肝癌细胞株H_(7402)产生NO和LPO的影响

Effect of direct lytic factor from Naja naja atra venom on nitric oxide and lipid peroxides produced by human liver cancer cell line H 7402

目的：研究眼镜蛇毒直接溶解因子（ＤＬＦ）对人肝癌细胞株Ｈ７４０２产生一氧化氮（ＮＯ）和脂质过氧化物（ＬＰＯ）的影响。结果：ＤＬＦ对体外培养的Ｈ７４０２细胞有杀伤作用，ＩＣ５０为２４７ｍｇ·Ｌ－１，ＤＬＦ与Ｈ７４０２细胞的生长抑制率呈量效关系。ＤＬＦ可介导Ｈ７４０２细胞产生大量ＮＯ２，ＮＯ２量与ＤＬＦ的剂量呈量效关系。在一定剂量范围内ＮＯ２量与Ｈ７４０２的生长抑制率呈显著正相关。用５７０型粘附式细胞仪测定单个细胞内ＬＰＯ，发现ＤＬＦ可使Ｈ７４０２细胞内ＬＰＯ产生增加。结论：ＤＬＦ可杀伤Ｈ７４０２细胞，其部分机制可能是通过诱导肿瘤细胞产生ＮＯ和ＬＰＯ。

AIM:To examine the effect of direct lytic factor (DLF) from Naja naja atra venom on nitric oxide (NO) and lipid peroxides (LPO) produced by human liver cancer cell line H 7402 RESULTS: DLF was cytotoxic on H 7402 cells cultured in vitro ,IC 50 was 2.47mg·L -1 .Growth-inhibiting rate of H 7402 in response to DLF was dose-dependent. As a stable metabolite of NO, nitrite production in culture supernatants was used to represent the level of NO. A large amount of NO 2 was produced by H 7402 cells which were cultured with DLF, production of NO 2 by H 7402 cells in response to DLF was dose-dependent. Close positive correlation was found between the level of ON 2 and growth-inhibiting rate of H 7402 in a certain range. Measuring the level of LPO in single cells production of LPO in H 7402 cells was increased by DLF CONCLUSION:Human liver cancer cell line H 7402 was damaged by DLF, the mechanism might be partly due to inducing the tumor cells to produce NO and LPO. .