缺血预处置对大鼠肢体缺血再灌注损伤的影响

The effect of ischemic preconditioning on the limb ischemia-reperfusion injury in rats

目的和方法在大鼠肢体缺血再灌注（ＩＲ）损伤模型上观察了缺血预处置（ｐｒｅｃｏｎｄｉｔｉｏｎｉｎｇ，ＰＣ）的影响。结果：发现ＰＣ可以明显减轻ＩＲ引起的血压降低，血浆乳酸脱氢酶、组织蛋白酶Ｄ活性及脂质过氧化产物丙二醛含量的升高；并抑制骨骼肌组织水肿及骨骼肌细胞线粒体钙超载的发生。同时还观察到，ＰＣ对ＩＲ时肢体微血管通透性升高、中性粒细胞浸润及血浆内皮素水平增高均有抑制作用。结论：ＰＣ对大鼠ＩＲ肢体有保护作用，其保护机理之一与其对血管床的保护作用有关。

AIM and METHOD:On the model of limb ischemia-reperfusion(IR),the effect of ischemic preconditioning(PC)were observed.RESULTS: After the rats' limb were subjected to IR,hypotension occurred and enzymes leaved out from the cytoplasma and lysosomes.In the plasma and tissue the malondialldehyde(MDA,the metabolite of lipid peroxides) content increased significally.The edema of tissue and overload of calcium in skeletal muscular mitochondria occured.At the same time,it was found that the capillary permeability increased and the neutrophil infiltrated into limb tissue.In the plasma,the endothelin level also increased .However,treatment with PC to IR rats can alleviate there changes as mentioned above.CONCLUSION: PC protected rats from limb IR injury and the protective action on capillary bed is one of the protective mechanisms of PC. .