TNF_α对人外周血CD_(15)^+细胞表面粘附分子的诱生及氨茶碱的作用

The inductive effect of TNF α on cellular adhesion molecules in human peripheral CD 15 + cells and the effects of aminophylline on it

目的和方法：应用双色免疫荧光流式细胞技术观察了肿瘤坏死因子（ＴＮＦα）对人外周血中性粒细胞（ＣＤ１５＋细胞）粘附分子ＣＤ１１α、ＣＤ１１ｂ、ＣＤ４９ｄ和ＣＤ６２Ｌ的诱生作用和氨茶碱对其影响。结果：不同浓度的ＴＮＦα可诱导中性粒细胞ＣＤ１１ａ和ＣＤ６２Ｌ的表达，对ＣＤ１１ｂ和ＣＤ４９ｄ无明显作用。不同剂量的氨茶碱对粘附分子的表达有一定作用。氨茶碱在７５ｍｇ／Ｌ浓度时增强ＣＤ１１ａ和ＣＤ６２Ｌ的表达；１２５ｍｇ／Ｌ时抑制其表达；２５０ｍｇ／Ｌ时抑制ＣＤ６２Ｌ的表达，对ＣＤ１１ａ无明显作用。结论：ＴＮＦα可调节中性粒细胞表面某些粘附分子的表达，氨茶碱对粘附分子的作用在临床应用上具有一定的指导意义。

AIM AND METHODS: The inductive effect of tumor necrosis factor (TNF α )on adhesion molecule expression (CD 11a ,CD 11b ,CD 49d ,CD 62L )in human peripheral neutrophils and the effect of aminophylline on it were investigated. The adhesion molecule expression was detected by bicolor immune labelling flow cytometry.RESULTS:The different dosage of TNF α promoted the expression of CD 11a and CD 62L to some extent,but did not have marked effect on CD 11b and CD 49d ,different dosage of aminophylline exerted different impact on adhesion at molecule expression,as indicated by increased CD 11a and CD 62L expression 75 mg/L concentration,decreased CD 11a and CD 62L and CD 62L expression at 125mg/L concentration,while no noted changes at 250mg/L concentration.CONCLUSION:The expression of some of the adhesion molecules were modulated by TNF α. The effects of aminophylline on adhesion molecule expression are of great importance in clinical work .