PCR检测大肠癌YNZ22位点VNTR的杂合性丢失

Detection of loss of heterozygosity at YNZ22 locus containing VNTR segment in colorectal carcinomas by polymerase chain reaction

目的：探讨ＹＮＺ２２位点串联重复序列（ＶＮＴＲ）的杂合性丢失在大肠癌发生发展中的作用。方法：应用多聚酶链反应技术对４１例大肠癌ＹＮＺ２２位点的ＶＮＴＲ区进行了分析。结果：大肠癌ＹＮＺ２２位点杂合缺失（ＬＯＨ）率为４３．３％。分析ＬＯＨ与临床病理参数间的关系发现，大肠癌ＹＮＺ２２位点杂合缺失与肿瘤大小，组织学类型，淋巴结转移，浸润深度和临床分期无关。结论：ＹＮＺ２２位点杂合缺失参与了大肠癌发生与发展。

ims: To evaluate the role of loss of heterozygosity(LOH)at YNZ22 locus in the development of colorectal carcinomas. Methods: Polymorphic analysis of YNZ22 locus that contains a variable number of tendem repeat (VNTP) was performed in 41 surgically resected specimens of colorectal carcinomas by the polymeras chain reaction(PCR). Results: Loss of heterozygosity(LOH)at YNZ22 locus was detected in 43.3% of informative cases. No sigificant difference existed between LOH at YNZ22 and tumor histological types, size, invasion, lymphnode metastasis and Dukes'stages(P>0.05).Conclusion: LOH at YNZ22 locus may be involved in carcinogenesis of colorectal cancer.