重组睫状神经营养因子对胶质瘤细胞系c-myc mRNA表达的影响

Effects of recombinant ciliary neurotrophic factor on cmyc mRNA expression of glioma cell line

目的：观察重组睫状神经营养因子（ｒＣＮＴＦ）对大鼠胶质瘤Ｃ６细胞系体外生长和癌基因ｃｍｙｃｍＲＮＡ表达的影响。方法：用细胞培养和Ｎｏｒｔｈｅｒｎ杂交技术检测ｃｍｙｃｍＲＮＡ的表达。结果：２００ｎｇ／ｍｌｒＣＮＴＦ可抑制Ｃ６细胞的增殖，细胞形态发生明显改变，ｃｍｙｃｍＲＮＡ的表达降低。结论：ｒＣＮＴＦ对Ｃ６细胞的生长抑制作用可能与其参与癌基因的调节有关。

?Purpose: To observe the effects of recombinant ciliary neurotrophic factor (rCNTF) on the growth and the cmyc mRNA expression of rat glioma C6 cell line. Methods: Cell culture and Northern blot techniques were used in glioma C6 cells to detect the morphological changes and the expression of cmyc mRNA Results: rCNTF 200ng/ml could inhibit the proliferation of C6 cell line, with distinctive morphological changes in the cells, and decrease the expression of cmyc mRNA. Conclusions: The effects of rCNTF on the growth of rat glioma C6 cells might involve an unknown regulative mechanism for the expression of oncogenes, and it may play a potential role as a cellular regulator in the treatment of glioma.