食管鳞癌人乳头瘤病毒DNA的检测研究

PCR in detdction of human papillomavirus DNA in esophageal carcinoma in xing jiang

目的：对新疆食管鳞状细胞癌中进行人乳头瘤病毒（ＨＰＶ）ＤＮＡ检测。方法：采用多重引物聚合酶链反应（ＰＣＲ）技术。结果：新疆５个地区４个民族１０４例食管鳞癌中ＨＰＶＤＮＡ阳性者占５０．９６％（５３／１０４），其中ＨＰＶ１６型为４９．０６％（２６／５３），ＨＰＶ１８型５．６％（３／５３），ＨＰＶ６／１１型７．５％（４／５３）；１６型和１８型同时阳性者２０．７５％（１１／５３），１８型和６／１１型１１．３２％（６／５３），１６型、１８型和６／１１型三者同时阳性５．７％（３／５３）；两个或三个类型的混合感染占３７．７％（２０／５３）。５个地区的食管鳞癌中均有不同程度的ＨＰＶＤＮＡ感染，４个民族中以哈萨克族食鳞癌ＨＰＶ感染率较高（８０．４８％）。结论：新疆地区食管鳞状细胞癌中ＨＰＶ有较高的感染率，以１６型为主，伴随与其它类型的混合感染，哈萨克族食管癌的发生发展与ＨＰＶ具有密切关系。

Aims:To detect the human papilloma virus in esophageal squamous cell carcinoma in Xin Jiang.Methods: Using multiple PCR.Results: A total of 104 human esophageal squamous cell cancinoma specimens in Xin Jiang from five areas and four nations. Fifththree(50.96%) of the 104 cases were positive for HPV DNA. HPV16 DNA was found in 26(49.06%) of the 53 cases, HPV 18 DNA in 3(5.6%) and 6/11DNA in 4(7.5%),but HPV 18 or 6/11 and 16 and all three mixpositive in 20(37.7%)were obsereve. The infection in Kazakh's esophageal carcinoma was the hightest(80.4%).Conclusions:HPV may be a possible etioligical agent for esophageal carcinoma in XinJiang.