补充硒减轻5-氟尿嘧啶对大鼠的脂质过氧化损害

Supplymentation of selenium reduces lipoperxidative damage by 5 fluorouracil in rats

目的：探讨补充硒以减轻５－氟尿嘧啶（５－ＦＵ）对大鼠脂质过氧化损害的机制。方法：６０只大鼠随机分为补硒组（Ｓｅ）和非补硒组（ＮＳｅ），经腹腔注射５－ＦＵ后，分别于注射前及注射后第１、３、５天处死，检测大鼠血液、心、肝、肾及空肠粘膜内丙二醛含量及谷胱甘肽过氧化物酶活性。结果：在Ｓｅ组，血中及空肠粘膜内丙二醛含量在注射５－ＦＵ后第１、３天，心、肝、肾组织内丙二醛含量在注射５－ＦＵ后第３天显著低于ＮＳｅ组；红细胞谷胱甘肽过氧化物酶活性在注射５－ＦＵ后一直显著高于ＮＳｅ组（Ｐ＜０．０１），空肠粘膜在注射５－ＦＵ后第１天，心、肝、肾组织内在注射５－ＦＵ后第３天，谷胱甘肽过氧化物酶活性显著高于ＮＳｅ组（Ｐ＜０．０５）。结论：补充硒有助于减轻５－ＦＵ对大鼠的脂质过氧化损害。

Objective:To evaluate the mechanisms of supplymentation of selenium(Se) reducing lipoperoxidative damage by 5 fluorouracil(5 FU) in rats. Methods:Sixty Sprague dawely rats were randomly divided into Se group and NSe group.After received intraperitoneal injection of 5 FU,they were sacrificed before( n =6) and on day 1,3 and 5( n =8) after injection respectively for the measurement of malondialdehyde(MDA) content,glutathione peroxidase(GGSH Px) activity in blood,heart,kidney,liver and jejunal mucosa. Results:MDA content in blood,heart,kidney,liver and jejunal mucosa was significantly lower in the Se group than in the NSe group on day 3,whereas it was also significantly lower in the Se group than in the NSe group in blood and jejunal mucosa on day 1 after injection.Erythrocyte GSH Px activity was significantly higher in the Se group than in the NSe group at different times.GSH Px activity in jejunal mucosa on day 1,and in heart,liver and kidney on day 3 was significantly higher in the Se group than in the NSe group after injeciton. Conclusion:Supplymentation of Se may reduce lipoperoxidative damage by 5 FU in rats.