放射酶分析法检测红细胞胰岛素降解酶活性

The determination of insulin degrading enzyme activity withradioassay of enzyme activity

本文采用放射酶分析法检测红细胞胰岛素降解酶活性（ＥＩＤＥＡ）。该方法应用方便、灵敏度极高、结果准确。并对酶反应最佳条件进行了实验探讨，底物Ｋｍ为０．０９ｍＵ／ｍｌ，最适ｐＨ值７．４０，延滞期２５分钟，２５～４０分钟测得ＢＩＤＥＡ基本一致。批内ＣＶ为３．０的，批间ＣＶ为３．３％。氯化物、ＥＤＴＡ可抑制该酶活性。５０例健康人红细胞胰岛素酶活性为８０，１８±１３．８０Ｕ／ｍｇＨｂ，无性别、年龄差异。

This paper described the method of radioassay of insulin. degrading enzyme activity of erytrocytes(EIDEA) It is simple, sensitive and accurate Optimum reaction conditions for EIDEA were investigated, The substrate Km was 0.09mU/ml. The optimum pH was 7.40.The delay time wits 25 minutes. The results of dctcrmination from 25 to 40 minutes were basically identical Within - run and between - run preeision were 3.0% and 3.3%,respeetively. Chloride and BDTA can inhibit EIDBA. The mean EIDEA of 50 healthy subjects was 80.18±13.80mU/mlHb.There was no sex or age difference.