PCR热启动及稀释法与常规法检测血清HBV-DNA的结果比较

The detection for serum HBV - DNA by polymerase . chain reaction(PCR) , hot start(HS)PCR and HS - PCR after dilution

采用常规ＰＣＲ、热启动ＰＣＲ、稀释后热泪动ＰＣＲ三种方法，对经ＥＬＩＳＡ检测为ＨＢｓＡｇ、抗ＨＢｃ、ＨＢｅＡｇ或抗ＨＢｅ阳性的血清标本，检测其乙型肝炎病毒ＵＮＡ（ＨＢＶ－ＤＮＡ）。结果显示热启动ＰＣＲ、稀释后热启动ＰＣＲ能提高ＨＢＶ－ＤＮＡ的阳性检出率。

Using Polymerase Chain Reaction(PCR) , Hot Start(HS) PCR. and HS - PCR after dilution , the serum HBV - UNA was detected, which has been proved to be positive for HBsAg, HBcAg, HBeAg or HBeAb in ELLSA . The results indicated that HS-PCR, HS - PCR after dilution could improve the positive detedtion rate of HBV - UNA.